鳜鱼基因表达转录分析中内参选择比较.PDFVIP

动 物 学 研 究 2010，Apr. 31(2) ：141−146 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI ：10.3724/SP.J.1141.2010.02141 Selection of Reference Genes in Transcription Analysis of Gene Expression of the Mandarin Fish, Siniperca chuasti 1,2 1,2 1 1 2 1 ZHOU Rui-Xue , MENG Tao , MENG Hai-Bo , CHENG Dun-Xue , BIN Shi-Yu , CHENG Jia , FU Gui-Hong, CHU Wu-Ying 1,* , ZHANG Jian-She1,* (1. College of Biological Engineering and Environmental Sciences, Changsha University, Changsha 410003, China; 2. College of Life Science, Guangxi Normal University, Guilin 541004, China) Abstract: At present，transcription analysis of gene expression commonly uses housekeeping genes as control for normalization ．In this study, the expression levels of three housekeeping genes including GAPDH, β-actin, and 18S rRNA in six tissues and five developmental stages of the Mandarin fish Siniperca chuatsi were assayed with quantitative real-time PCR (qPCR) ．Differences in expression levels were analyzed using geNorm program. The results demonstrate that β-actin is the most stable gene at developmental stages and GAPDH is the most stable in different tissues. While 18S rRNA expression during development is differentially regulated, which indicates it is suitable as an internal control for gene expression normalization at the developmental level. Overall, the data suggest that the two most stable housekeeping genes are enough to accurately calibrate gene expression in S. chuatsi. The significance of this study provided convincing references and methodology f