酶底物法产品培训.pptVIP

固定底物技术酶底物法 科立得 －所需试剂及设备 定性 科立得试剂 取样瓶（可用玻璃瓶替代） 定量 科立得试剂 取样瓶（可用玻璃瓶替代） 51孔/97孔定量盘 程控定量封口机 * Colilert has a far lower false positive rate than MF. According to the US EPA Federal Register, July 17, 1989 page 29998 Column #3, membrane filtration’s false positive rate (22%) is 69% higher than Colilert’s (13%). Nearly double! Comparison of Membrane Filtration and Autoanalysis Colilert Presence-Absence Techniques for Analysis of Total coliforms and Escherichia coli in Drinking Water Samples (Carrie M. Lewis and Jeannie L. Mak, Applied And Environmental Microbiology, Dec 1989) found that MF’s total coliform false positive rate was 136% higher than Colilert’s. In MF, 12 of 23 (52%) metallic sheen colonies could not be verified. In Colilert, only 2 of 9 (22%) samples where yellow color was observed could not be verified in LTB and BGLB. “Use of Two Formulations of Colilert and Quanti-Tray for Assessment of The Bacteriological Quality of Water”, Fricker et al, Water Res. 1997, study of 7,389 drinking water samples showed 29% (106/363) of the UK’s MF (MLSB) positives don’t confirm and all of Colilert’s 296 coliforms confirmed. Nation Field Evaluation of a Defined Substrate Method for the Simultaneous Enumeration of Total Coliforms and Escherichia coli from Drinking Water: Comparison with MTF Method (Edberg, Allen Smith, Applied and Environmental Microbiology, June 1988) found no false positives due to heterotrophs at extremely high microbial concentrations (2 million CFU/100ml). Also, isolates of Pseudomonas and Flavobacterium species did not demonstrate false positives at concentrations up to 5 million CFU/100 ml. Evaluation of the Autoanalysis Colilert Test for Detection and Enumeration of Total Coliforms (Covert et al. Applied and Environmental Microbiology, October 1989) found levels of heterotrophic bacteria normally found in drinking water did not interfere with detection or enumeration of coliforms in Colilert. Colilert is refractory