锦州市手足口病毒核酸检测的的结果分析.docVIP

锦州市手足口病毒核酸检测的的结果分析 　　摘要：目的 分析锦州市手足口病病原学检测结果，为临床手足口病防治提供依据。方法 选取100例2015年锦州市临床临床诊断为手足口病病例的咽拭子标本，提取样本核酸，用RT-PCR方法检测柯萨奇病毒A16型（CA16）、肠道病毒71型（EV71）、和非EV71、CA16的其他肠道病毒（EV）。结果 2015年3～12月份共采集手足口病病例标本100份。核酸检测结果显示，阳性标本86例（86.00%）；在2015年3～12月均有手足口病患者出现，3～5月份病例数急剧增加，5月份为最高峰，阳性检出率为45.35%，6～12月份病例数逐渐下降；男孩核酸检测阳性率显著高于女孩（P0.05）；在不同年龄组之间，1岁组，EV71、EV阳性数构成比均为年龄组中最高。结论 锦州市2015年3～12月份均有手足口病发生，病原以EV71感染为主，在不同月份、年龄、性别上发病率存在一定差异。 　　关键词：手足口病；柯萨奇病毒A16型；肠道病毒71型 　　中图分类号：R440 文献标识码：A 文章编号：1006-1959（2017）21-0184-02 　　Analysis of Nucleic Acid Test Results of Hand-Foot-Mouth Virus in Jinzhou City 　　YANG Fei 　　（Jinzhou Disease Prevention and Control Center，Jinzhou 121000，Liaoning，China） 　　Abstract：Objective To analyze the results of hand foot mouth disease in Jinzhou city，provide the basis for clinical prevention and treatment of HFMD.Methods 100 cases of clinical diagnosis in Jinzhou city in 2015 HFMD cases of throat swab specimens，samples of nucleic acid，RT-PCR was used to detect Coxsackie virus type A16（CA16），enterovirus 71（EV71），and non EV71，other intestinal virus CA16（EV）.Results From March to December in 2015 were collected 100 samples of HFMD cases.Nucleic acid detection results showed that the positive specimens of 86 cases （86.00%）；From March to December in 2015，there were hand，foot and mouth disease patients，the number of cases increased from March to May，the highest peak in May，the positive detection rate was 45.35%，from June to December the number of cases were decreased；the positive rate of boys was significantly higher than that of nucleic acid detection girl（P0.05）；among different age groups，1 years old group，EV71，the constituent ratio were the highest in EV age group.Conclusion Hand foot mouth disease occurred in Jinzhou from March to December in 2015.The main cause of infection was EV71 infection，and there were some differences in age，sex and incidence in different months. 　　Key words：Hand，foot and mouth disease；Coxsackie virus A16；Enterovirus 71 type 　　手足口病是多?N病毒引起的，患者主要