《堆型艾美耳球虫鸡胚培养研究》-毕业学术论文设计.docVIP

PAGE 精品 精品 河北北方学院毕业论文 堆型艾美耳球虫鸡胚培养研究 Studies on chick embryo of Eimeria acervulina culture 学 生: 指导教师: 院 系: 动物科技学院动物医学系 专 业: 动植物检疫 班 级: 动植物检疫本科班 论文提交日期：2011年6月9日 目录 引言·······································································3 1.1材料···································································5 1.1.1实验动物·····························································5 1.1.2试验虫株·····························································5 1.13试验药品和试剂························································5 1.2方法···································································5 1.2.1卵囊的收集与纯化··················································5 1.2.2子孢子制备·························································5 1.2.3鸡胚接种··························································5 1.2.4卵囊计数及收集····················································6 2结果·····································································6 2.1鸡胚接种日龄·························································6 2.2鸡胚接种剂量···························································6 3讨论·····································································7 参考文献···································································9 致谢······································································11 堆型艾美耳球虫鸡胚培养研究 魏亚然 （河北北方学院动物科技学院动物医学系） 指导教师：李忠浩 摘要：目的:建立堆型艾美耳球虫的鸡胚培养体系，为以后的研究作参考。 方法:用堆型艾美耳球虫子孢子分别接种8-11日龄鸡胚以确定最佳接种日龄，用不同剂量子孢子接种同一日龄鸡胚以确定最佳子孢子接种剂量。 结果:用堆型艾美耳球虫子孢子接种9日龄的鸡胚，其卵囊产量和卵囊成熟率均为最高，成熟率达99%;当每个鸡胚接种子孢子1×104个时，其卵囊产量最高。 结论:本文通过对堆型艾美耳球虫的鸡胚接种日龄﹑子孢子接种剂量两方面因素的研究，初步建立了堆型艾美耳球虫的鸡胚培养体系，为日后确定合适的接种日龄和接种剂量提供可靠的数据。 关键词：堆型艾美耳球虫 鸡胚 接种日龄 接种剂量  Studies on chick embryo of Eimeria acervulina culture （Wei Ya-ran） (Veterinary Medicine,Colloge of Animal Science and Technology, Northern University of Hebei) Guide Teacher:Li zhong hao Abstract: Objective: E