海洋链霉菌GB-2抗细菌物质的溶解性质和分离纯化.PDFVIP

306 2008, Vol. 29, No. 07 食品科学 ※生物工程 陆颖健，董 昕，刘 姝，别小妹* (南京农业大学食品科技学院，江苏南京 210095) ：本实验主要对海洋链霉菌株GB-2 产生的抗菌物质的溶解性进行了研究，并将其分离纯化。通过对GB- 2 的发酵液中抗菌物质的溶解性测定，可推知是一种极性较大的水溶性物质。超滤实验发现，抗细菌组分能通过 截留分子量(COMW) ≤1kD 的超滤膜。Sephadex LH-20 色谱柱分离后，经抗菌活性检测发现两个活性峰，其中第 1 个峰对蜡样芽孢杆菌和大肠杆菌的抑菌活性最强。将第1 个峰的收集液进一步用高效液相色谱(HPLC)纯化，在图 谱上出现两个峰，其中第2 个峰具有抑菌活性，表明该物质得到了纯化。 ：海洋链霉菌G B - 2 ；抗细菌物质；溶解性；分离和纯化 Solubility, Separation and Purification of Antibacterial Substances Derived from Marine Streptomyces sp. GB-2 LU Ying-Jian ，DONG Xin ，LIU Shu ，BIE Xiao-Mei* (College of Food Science and Technology , Nanjing Agricultural University , Nanjing 210095, China) Abstract: The solubility and purification of antimicrobial substances produced by Streptomyces sp. GB-2 were studied. By determination, it was deduced that the antibacterial substances in GB-2 broth has water solubility property with polarity. They can pass through the ultrafiltration membrane with cut off molecular weight (COMW) 1 kD. By separation on the Sephadex LH- 20, two peaks with antibacterial activity appear, in which the first peak displays the strongest inhibition activity against Bacillus cereus and E.coli . In order to further purify the fraction, a high performance liquid chromatography (HPLC) separation was performed. The results showed that the fraction comprises two peaks and the second peak was found to be of antibacterial activity. Key words ：marine Streptomyces GB-2 ；antibacterial substances ；solubility ；separation and purification 中图分类号：TS201.3 文献标识码：A 文章编号：1002-6630(2008)07-0306-05 从自然资源中筛选新型抗菌物质依然是寻找广谱高 明了其的杀菌效力和无副作用，这个研究发现表明自然 效、安全无毒、将来能替代现在使用的化学防腐剂和 界中还有许多新物质等待我们去发现，这进一步促进了 作为饲料添加剂的抗菌