Resveratrl对人外周血T细胞活化的影响及机制研究.docVIP

Resveratrol对人外周血T细胞活化的影响及机制研宄 夏菲邹婷张进平(通讯 ) (苏州大学生物医学研究院215123) 】目的木研究旨在探讨Resveratrol对人T淋巴细胞活化的影响及 其分子机制。方法在体外使用Resveratrol处理人外周血T细胞，并给予抗人 CD3和抗CD28抗体活化，观察其对T细胞增殖、细胞因子分泌及细胞凋亡的影 响。同时进一步通过免疫印迹和免疫共沉淀的方法?探讨Resveratrol对T细胞活化 影响的分子机制。结果我们现有的实验提示Resveratrol可以明显抑制人外周血 T淋巴细胞的增殖，且能抑制IL-2、IL-4、IL-5、IFNgamma;等细胞因子的分泌; 但并不影响T细胞的凋亡。进一步研究表明Resveratrol通过提高Sirtl的表达和 增强其对C-jun的脱乙酰化来发挥作用。结论此研究提示resveratrol司*能在.T 细胞引起的炎症如自身免疫性疾病治疗中发挥重要作用。 关键词】Resveratrol T细胞活化脱乙酰化 C-jun Sirtl 】R392 】A 】2095-1752 (2014) 02-0152-03 The Roles and Molecular Mechanisms of Resveratrol on Human T cell Activation XIA Fei, ZOU Ting, ZHANG Jin-ping (Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123 Jiangsu Province, China) [Abstract] Object: We aim to explore the function and molecular mechanisms of resveratrol on human T cell activation. Method: The PBMC were obtained from health people, and purified by Ficoll-hypaque, the T cell were activated by anti-CD3 and anti-CD28 antibodies, the proliferation was evaluated by CFSE cell division assay and Brdu cell proliferation assay. The levels of cytokines in activated T cells culture supernatant were measured by ELISA. The expression of Sirtl and alteration of acetylation of C-Jun were evaluated by western-blot. Result:We found that Resveratrol significantly inhibited the activation and cytokines production of T cell in hunman PBMC. The expression of Sirtl was up-regulated and acetylation of C-Jun was decreased in resveratrol-treated T cells. Conclusion: Resveratrol inhibited the T cell activation by up-regulating the expression of Sirtl and enhancing the deacetylase activity of Sirtl on C-Jun in T cells. Keywords】 Resveratrol Sirtl T cell activation deacetylase C-Jun Resveratrol是一种天然的多酿化合物，富含在桑椹、花生、葡萄等植物中［1, 2］。自从人们发现饮用红葡萄酒的人能抵抗冠心病以来，Resveratrol得到了科学 家的广泛关注［3】。很多文献报道Resveratrol在炎症、氧化、癌症以及衰老中发 挥重要作用［4-8］。Subhash C.Gautam和同事体外实验研究发现Resveratrol能抑制 ConAJL-2以及异体抗原对脾脏细胞的增殖［9］。五年后，Agrewala等人研究发现， Resveratrol可以抑制T细胞、B细胞、巨噬细胞等增殖、抗体产生、和淋巴因