丙泊酚对糖尿病S大鼠局部脑缺血再灌注损伤保护作用的研究.docVIP

丙泊酚对糖尿病SD大鼠局部脑缺血再灌注损伤保护作用的研宄 孙洋崔岚宫兵(黑龙江省医院150036) 屮图分类号】R972 】A 】1672-5085 (2012) 16-0075-03 】FI的采用糖妞病Spngue-DawleWSD)大鼠局灶性脑缺血再灌注模型，观 察神经功能缺损评分、脑梗死体积、脑组织病理形态改变情况，探讨预防性应用 丙泊酚对糖尿病SD大鼠局灶性脑缺血再灌注损伤是否有保护作用。方法50只 雄性健康sD大鼠，腹腔注射链脲佐菌素(strepozotocin，STZ)55 mg / kg复制糖似 病人鼠模型，72h后剪尾尖采血测定血糖，以后每周测血糖，血糖持续 ge;16. 7mmol/L为糖尿病大鼠(50只人鼠造模成功36只)。36只糖尿病人鼠， 建立高血糖模型后随机分为2组：高血糖组(n=18＞、丙泊酚+高血糖组(简称丙泊 酚组n=18)，各组按脑缺血90 min再灌注3 h(n=6)、6h(n=6)、24h(n=6｝分为3个 亚组。采用线栓法制作人脑屮动脉栓塞模型。比较丙泊酚组与高血糖组各再灌注 时间点神经功能缺损评分、脑梗死体积和脑组织病理形态的改变。结果相同再 灌注时间点，丙泊酚组比高血糖组神经功能缺损程度减轻，Plt;O. 05；相同时 间点丙泊酚组较高血糖组梗死体积缩小，其屮再灌注3、6 h组间比较Plt;0.05, 再灌注24 h组间比较Plt;O. 01。结论预防性应用丙泊酚能减轻高血糖条件不 的局灶性脑缺血再灌注损伤，减轻经功能缺损症状，缩小梗死体积。 关键词】丙泊酚糖尿病脑缺血再灌注 Effects and Mechanisms of Propofol on Focal Cerebral Ischemia / Reperfusion Injury in Diabatic Rats Abstract 】Objective Applying focal ischemia—reperfusion model of SD rat on the condition of hyperglycemia, through observing the state of neurologic impairment score, cerebral pathomorphology and infarction volume after focal ischemia-reperfusion damage in SD rat on the condition of hyperglycemia. Method： 1)50 male healthy rats,weighing 180 to 220 grams, making the diabeticanimal model by intraperitoneal injection streptozotocin(produced by sigmaeompany). If the random blood sugar higher or equal 16. 7mmol / L Was regarded as success of diabetic model three days later, then raised the rats for 6 to 7 weeks(36succesfull). All the rats were divided 2 groups： hyperglyeemla group(n=18)and propofol+hyperglycemia group(namely propofol group n=18). Each group wag divided into 3 subgroups according to reperfusion 3 h(n=6), 6 h(n=6)，24 h(n=6)ofter ischemia for 90 minutes. Compare the differences of neurologie impairment score, cerebral infarction volum and pathomorphology. Results 1- There are great difference of the neurologic impairment score between the propofolgroup and hyperglycemia group(Plt;0.05)，The infarction volume can he seen at propofol group，and the peak Wag at reperfusion 24 hours. Compared 6 h wi