抗弓形虫水通道蛋白多肽抗体的制备及鉴定-OpenRepositoryof.PDFVIP

Chinese Journal of Zoonoses DOI 10 3969 issn 1002 2694 2015 10 002 j TgAQP M E TgAQP B KL H ELISA Western blot KL H ELISA Western blot kDa TgAQP R382 5 A 1002 2694 2015 10 0903 05 Preparation and identification of anti TgAQP peptide antibody ZHANG Jiafeng HAO Wenbo XIAO Bing LIAO Xiao ing LUO Shuhong q Institute of A ntibod y Eng ineering College of Biotechnology Southern Medical University Guangz hou China Abstract In this study we intended to prepare antiToxo lasma ondii aquaporin TgAQP eptide antibody which was p g p used to the application in the detection of the aquaporin expression and its subcellular localization of Toxop lasma gondii T ondii M E strain The B cell peptide antigen was designed based on the TgAQP amino acids sequence After the pep g tide antigen was conjugated to the KL H the fusion antigen was injected into New Zealand rabbits to prepare polyclonal anti body followed by identification of ELISA Westernblotting and immunofluorescence assays The ELISA showed that the titer of antiTgAQP antibody was about Western blotting revealed the specific affinity of the antigen to polyclonal anti body at kDa protein T ondii The protein detected by the indirect immunofluorescence assays was distributed in the cy g toplasm of the parasite Thus far the antiTgAQP polyclonal antibody was successfully prepared roviding a useful tool for p further study of biological function and metabolic characteristics of TgAQ