Neurobiotin, a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal tract-tracing and for in vitro labeling of neurons-学术期刊.pdf

.hmrmd ol Neuroscience Method.~, 41 (1992) 31 43 3 I ~, 1992 Elsevier Science Publishers B.V. All rights rcservcd (1165-0270/92/$05.00 NSM 01318 Neurobiotin TM a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal tract-tracing and for in vitro labeling of neurons Q i n H u a n g , D a n Z h o u a n d M a r i a n D i F i g l i a l,ahoratory of (elluhlr N¢urohiolow, Department of Neurolo~,3, Massachtcsett.~ (;emral tto,v~ital mul llarcard Medical ,~chool, Boston, MA 02114 (U.S.A.) (Received 9 July 1991) (Reviscd version received 2(I September 1991) (Accepted 26 September 1991) Key words: B iot i n ; A v i d i n ; N e u r o b i o t i n t M ; B a s a l g a n g l i a ; C e r e b r a l cortex; A n t e r o g r a d e t r a c i n g ; R e t r o - g r a d e t r a c i n g ; T r a n s n e u r o n a l t r a c i n g ; Cell c u l t u r e The biotin derivative, N-(2-aminoethyl) hiotinamide hydrochloride, o r Ncurobiolin TM, has hccn shown recently to be a useful marker for intracellular and anterogra