酶的磷酸化调节酵母的中心代谢.PDF

酶的磷酸化调节酵母的中心代谢 报告人：刘涛 一.简介 Saccharomyces cerevisiae 酿酒酵母 蛋白磷酸化：一种常见的翻译后修饰，调控细胞代谢、 渗透应力、生长、分裂等。 磷酸化的作用：激活或者抑制目标蛋白 1.改变蛋白构象 2.打开或封闭底物催化中心 3.决定物质转运 4.把蛋白和其他蛋白复合物结合在一起 5.标记要被降解的蛋白 二.研究焦点 1.The majority of functional phosphoenyzmes are still unknown. 2. Previous studies focused on the qualitative effect of protein phosphorylation without considering the impact of phosphorylation on the operation of the metabolic network as whole. 3.We quantitatively measured the phosphoproteome of S. cerevisiae during growth under five environmental conditions. 三.实验方法 We quantitatively measured the phosphoproteome of S. cerevisiae during growth under five environmental conditions. 三.实验结果 1. Phosphopeptide patterns 556 proteins with at least one phosphorylation site. Of the 681 enzymes contained in the reaction network of yeast metabolism, 70 with at least one phosphorylation site, 10 of which do not have a phosphorylation site yet listed in PhosphoPep. 三.实验结果 2.Degree of phosphorylation 三.实验结果 3.The impact of phosphorylation on enzyme activity 三.实验结果 4.在代谢网络中酶磷酸化的功能相关性 glucose (black) galactose (red) anaerobic (green) ethanol (yellow) 三.实验结果 5.Functional impact of phosphosite removal on metabolic activity. phosphorylated serine residues non-phosphorylatable alanine 三.实验结果 三.实验结果 6.Identification of functional phosphorylation sites for Pfk2 and Gpd1 四.研究结论 1. 建立了一种在体内代谢网络水平上，研究酶的磷酸 化的新方法。 2. 对酵母