贵州地产蜘蛛香质量剖析.docVIP

贵州地产蜘蛛香质量剖析 　　[摘要] 目的:评价贵州省各地所产蜘蛛香的内在质量。方法:采用高效液相色谱法对蜘蛛香中橙皮苷进行含量测定;色谱柱为DiamonsilC18(200 mm×4.60 mm,5 μm),流动相为甲醇-醋酸-水(35∶4∶61);检测波长为283 nm,流速为1.0 ml/min,柱温为30℃。橙皮苷在0.077 18～0.848 76 μg范围内,进样量与峰面积呈良好线性关系,相关系数r=0.999 99(n=6);橙皮苷回收率为102.32%,RSD=1.95%(n=6)。结果:不同产地蜘蛛香中橙皮苷含量变化范围较大。结论:通过检测蜘蛛香中橙皮苷含量,可以为蜘蛛香内在质量控制提供参考数据,该方法简单、可行,结果准确、可靠。 　　[关键词] 蜘蛛香;橙皮苷;高效液相色谱法 　　[中图分类号] R927.2 [文献标识码]C [文章编号]1674-4721(2010)05(a)-046-02 　　 　　Quality analysis for Valeriana jatamansi Jones from different habitats in Guizhou 　　CHEN Ling, BAO Jiake, XU Hong, XIONG Huilin, CHEN Wanli 　　(Guizhou Provincial Institute for Drug Control,Guiyang 550004,China) 　　[Abstract] Objective: To appraise the quality for Valeriana jatamansi Jones from different habitats in Guizhou. Methods: By high performance liquid chromatography on Valeriana jatamansi Jones to determine the content of hesperidin;column was DiamonsilC18 (200 mm×4.60 mm,5 μm), mobile phase was methanol-acetic acid-water (35∶4∶61); detection wavelength was 283 nm,flow rate was 1.0 ml/min,column temperature was 30℃.Hesperidin in 0.077 18~0.848 76 μg range,the sample volume and the peak area was linear with a correlation coefficient of r=0.999 99(n=6);hesperidin recovery was 102.32%,RSD=1.95%(n=6). Results:Valeriana jatamansi Jones from different areas of hesperidin content varied greatly. Conclusion:By detecting the content of hesperidin in Valeriana jatamansi Jones for internal quality control of reference data,the method is simple,feasible, accurate and reliable. 　　[Key words] Valeriana jatamansi Jones; Hesperidin; HPLC 　　 　　蜘蛛香为败酱科植物蜘蛛香Valeriana jatamansi Jones的干燥根茎及根[1],蜘蛛香又名马蹄香、老虎七、印度缬草等,系败酱科缬草属植物。主要产于贵州、云南等地。临床主要用于消化不良、腹痛、止水泻、流感、镇静、安定等。橙皮苷(hesperidin)是蜘蛛香的主要成分之一,含量高、稳定性好,据报道[2-3],橙皮苷也具有镇静和催眠作用,是新发现的作用于中枢神经系统的黄酮类化合物,为控制蜘蛛香药材品质,对贵州不同产地蜘蛛香中橙皮苷进行了高效液相色谱法(HPLC)含量测定。 　　1 仪器与试剂 　　紫外可见分光光度仪:岛津UV-2550型分光光度仪;高效液相色谱仪:岛津LC-2010ATH液相色谱仪,LC-solution色谱工作站。色谱柱为DiamonsilC18(200 mm×4.60 mm,5 μm);橙皮苷对照品(110721-200512)购自中国药品生物制品检定所;蜘蛛香药材产自贵州省各地,经贵阳中