丹磺酰白藜芦醇抑制乳腺癌MCF-7细胞增殖的初步研究-药理学专业论文.docx

PAGE PAGE 10 preliminary study of dansyl resveratrol induced apoptosis to MCF-7 breast cancer Abstract Objective: To research the inhibiting effect of resveratrol dansyl(Resveratrol derivatives) on human breast cancer MCF-7 cell line in vitro; And to explore it,s mechanism in the process. Methods:To chemical synthesize the fluorescence derivatives of resveratrol dansyl.By MTT assay showed the proliferation on MCF-7 cell with resveratrol and resveratrol dansyl . Detecting apoptosis by flow cytometry . At the same time by the method of plate cloning showed cell morphology and apoptosis. Indirect immunofluorescence shows dansyl resveratrol co-localized with caveolin-1;The proteins expression of caveolin-1,P53,PTEN,MDM2 was determined by western blot. Results：MTT assay showed that resveratrol and resveratrol dansyl can inhibit MCF-7 cell proliferation, and resveratrol dansyl is higher than resveratrol .The method of plate cloning also showed that resveratrol dansyl can promote apoptosis of MCF-7 cells.Flow cytometry results show resveratrol dansyl can induce MCF-7 apoptosis, and the apoptosis rate increases with the increasing concentration.Chemical synthesis of resveratrol derivatives of resveratrol dansyl; With indirect immunofluorescence detection of intracellular RES and caveolin-1 distribution and localization, It was found that caveolin-1 and resveratrol dansyl have the same distribution area..Western blot results show that with the concentration addition of dansyl resveratrol the expression of caveolin-1,P53,PTEN protein increased; MDM2 downregulated. Conclusion：1.Resveratrol dansyl can inhibit the growth of MCF-7 cell, and have a strong anti-tumor activity. 2.RES-DAN can promote the expression of endogenous caveolin-1 and raised P53,PTEN expression and reduced the expression of MDM2. Keywords：Resveratrol dansyl;caveolin-1; MCF-7 cell; apoptosis. Yi Xiang (pharmacology) Directed by professor: Hui-ling Yang 前 言 乳腺癌是女性最常见的恶性肿瘤之一，是乳腺导管上皮细胞在种种内外致癌 因素的刺激作用之下，