法舒地尔下调哮喘气道炎症和抗氧化作用的机制研究-内科学专业论文.docx

但常规+法舒地尔治疗组前后治疗的血清 IL-3、IL-4、IL-17 和 TNF-α 浓度差值明显高于比常规哮喘组，并有统计学意义（P0.05）。结论： 1. 动物实验：哮喘小鼠通过上调哮喘相关因子和蛋白表达而诱发小 鼠气道高反应性、肺组织的炎症和氧化应激，从而引发一系列哮喘临 床症状。给予法舒地尔提前干预能在一定程度上减轻这一作用。2. 细 胞 实 验 ： 屋 尘 螨 可 诱 导 人 支 气 管 上 皮 细胞 （ 16 human bronchial epithelial cells, 16HBE）的氧化应激，上调相关炎症因子和蛋白表达， 导致细胞损伤。法舒地尔提前干预可减轻上述作用。也证明法舒地尔 有抗炎症和抗氧化应激的作用。3. 临床实验：法舒地尔能够减轻哮 喘患者血清中的哮喘相关炎症因子的浓度，再次证明法舒地尔能减轻 哮喘患者炎症反应。4. 综上说明 Rho 激酶-1 可能参与过敏原诱导的 哮喘气道炎症过程，Rho 激酶-1 抑制剂法舒地尔能够明显减轻哮喘 气道高反应性、抗炎和抗氧化应激作用。 关键词：哮喘；气道炎症；氧化应激；Rho 激酶抑制剂 The effect and mechanism of Fasudil on anti-inflammatory and anti-oxidative stress in asthma Abstract: Objective: Recent studies show that fasudil plays an important role in asthma, but the mechanism is still not very clear, so the experimental study of the effect of Rho-1 kinase inhibitor fasudil on anti-inflammatory and antioxidative in bronchial asthma (asthma), explore Rho kinase-1(ROCK-1) role in asthma. Methods : This experiment is divided into three parts: 1. animal experiment: 24 female mice(5~6 weeks) were randomly divided into control group.asthma group and fasudil group, with 8 rats in each group. asthma group and fasudil group intraperitoneal injection and intranasal sensitization and challenged With ovalbumin (OVA), fasudil group in nasal drops before usage of fasudil intervention, control group was given the same dose of normal saline. The groups were determination of airway responsiveness, bronchoalveolar lavage fluid (BALF) cell count, lung tissue HE staining and PAS staining, Immunoblotting detection of NF-κB.PNF-κB.STAT6. PSTAT6.TNF-α and Rho kinase-1, the expression level of MUC5AC was measured by immunofluorescence, expression of ROS in lung tissue of fluorescence probe -DHE detection in ROS. 2. Cell experiment: It was divided into control group, dust mite group and fasudil group, fasudil group was treated with fasudil (concentration of 45umol/l) for an hour, dust mite group and fasudil group at the same time were given house dust mite (concentration of 20ug/ml) for