雌激素受体及运动应激对小鼠睾酮CPP的影响民族传统体育学专业论文.docxVIP

中北大学学位论文 中北大学学位论文 皮下注射他莫西芬能够显著减弱睾酮形成的 CPP 作用（P 0.05）当单独给予他莫 昔芬时不能形成小鼠的 CPP。 4）运动应激对小鼠形成睾酮 CPP 的作用表达 运动应激后的小鼠与非运动后的小鼠 sc 睾酮训练后 CPP 得分值均明显高于空白对 照组（P 0.05）。但是运动应激与非运动的小鼠 sc 睾酮训练后 CPP 得分值没有明显差异 （P 0.05）。 结论： 睾酮各剂量均可以形成小鼠 CPP 的表达；雌激素受体拮抗剂 ICI182,780 和他莫西 芬均可以抑制小鼠睾酮 CPP 的表达；运动应激没有明显提高睾酮 CPP 的表达。说明睾 酮具有奖赏效应，雌激素受体可能参与其中。本文所使用运动应激方法可能没有直接对 睾酮的奖赏效应起作用。 关键词：睾酮；雌激素受体；拮抗剂；条件性位置偏爱；运动应激；小鼠 Effects of Estrogen Receptor and Exercise-induced Stress on Testosterone-induced CPP in Mice Abstract Testosterone is one of the anabolic androgenic steroids (AAS) derivatives, the most common drug in athletic events. In recent years, a series of animal experiments and clinical studies showed that the AAS has a reward and reinforcement effect which would produce physical and psychological dependence just as the classical drugs. However, compared with the classical opioid drug abuse, the study of the mechanism of AAS abuse is not clear. It wonders whether the exercise-induced stress affects the susceptibility of body and psychological dependence. Objective: This article used behavioral pharmacology methods, mainly studies on estrogen receptor in central nervous system and exercise-induced stress on testosterone-induced conditioned place preference in mice (CPP) roles. This article has certain significance in expounding AAS and AAS on the central role of the reward system behavioral pharmacology of abuse mechanism. Methods: The role of Estrogen receptor to the reward effect on testosterone used conditioned place preference test. First in order to determine testosterone formation in mice CPP best dose of mg·kg-1, 1 mg·kg-1, 2 mg·kg-1 mice were injected subcutaneously. Each 2d is a training cycle. Later, before training CPP gives estrogen receptor antagonist ICI182,780 or Tamoxifen (sc) forms testosterone CPP to conduct the effect test to the mice. To the movement stress training test experiment, mice were given testosterone (sc) after high-intensity treadmill training. 30min to carry on the CPP tr