丹栀逍遥散对D-氨基半乳糖所致大鼠急性肝损伤的保护作用中医基础理论专业论文.docx

PAGE PAGE 10 中 文 摘 要 目 的：探讨丹栀逍遥散对 D-氨基半乳糖所致大鼠急性肝损伤的保护作用 及可能的机制。 方 法：将 60 只 SD 雄性大鼠随机分为空白对照组、模型组、联苯双酯组、 丹栀逍遥散高、中、低剂量组，每组 10 只。连续灌胃给药 7 天，空白组和 模型组则给予等体积生理盐水，末次给药后 1 小时，除空白对照组腹腔注 射等体积生理盐水外，其余大鼠均按照 800mg/kg 腹腔注射 D-GaIN 造成肝 损伤模型，24h 后，腹主动脉取血，检测血清中谷草转氨酶（AST）、谷丙 转氨酶（ALT）、肝匀浆中超氧化物歧化酶（SOD）、还原型谷胱甘肽-过氧 化物酶（GSH-PX）、丙二醛（MDA）的含量。 结 果：造模 24h 后，模型组 ALT、AST、MDA 水平明显升高，SOD、 GSH-PX 明显降低，与空白对照组相比,差异有统计学意义(P0.05);丹栀逍 遥散各剂量组 ALT、AST、MDA 水平明显降低，SOD、GSH-PX 明显升高， 与模型组相比，其中大部分差异有显著性（P0.05）。 结 论：丹栀逍遥散对 D-氨基半乳糖所致的大鼠急性肝损伤具有保护作 用，其机制与抗过氧化程度有关。 关键词： 丹栀逍遥散；急性肝损伤；D-氨基半乳糖 Experimental Study of Protective Effects of Danzhixiaoyao powder on Acute Liver Injury Caused by D-galactosamine in Rats Abstract Objective: To investigate the protective effects of Danzhixiaoyao powder on acute liver injury caused by D-galactosamine in rats and to study the probable mechanism of protective effects. Methods: 60 male SD rats were randomly divided into control group, model group, positive medicine group, and low, middle, high dose groups of Danzhixiaoyao powder these 6 groups, 10 rats each group. treatment groups were filled with medicine for 7 days continuously, acontrol group and model group with the same amount of Physiological saline, 1 hour after the last lavage, intraperitoneal injection of D-GaIN at a dose of 800mg/kg was administered to the rats except those in the control group with the same content of saline. After 24 hours, the contents of AST, ALTin blood serum and the contents of SOD, GSH-PX, MDA in liver tissue plasm were measured by collecting blood from abdominal aorta. Results: 24 hours after made model, AST, ALT and MDA in model group were increased significantly, SOD and GSH-PX were decreased significantly, compared with the blank group, the difference was statistically significant, AST, ALT and MDA in each dose group of Danzhixiaoyao powder were decreased significantly, SOD and GSH-PX were increased significantly, compared with the model group, the difference was statistically significant. Con