丹皮酚衍生物的合成及其抗肿瘤活性分析-生物医学工程专业论文.docx

PAGE PAGE 6 Schiff base derivatives: N-[1-(2,4-dihydroxyphenyl)ethylid-ene]benzohydrazide (B1) Bi(2’,4’-dihydroxy-α- methylbenzylidene)-diimine (B2) Bi(2’,4’-dihydroxy-α-methylbenzyl-idene)-ethylenediimine （B3） Bi(2’,4’-dihydroxy-α-methylbenzyl-idene)-1,3-trimethylenediimine （B4） 5-methoxy-2-[-1-(propylimino)ethyl]phenol（P1） Bi(2’-hydroxy-4’-methoxy-α-methyl-benzylidene)-diimine （P2） Bi(2’-hydroxy-4’-methoxy-α-methyl-benzylidene)-ethylenediimine（P3） Bi(2’-hydroxy-4’-methoxy-α-methyl-benzylidene)-1,3-trimethylenediim-ine（P4） Metal complexes: CuB1、CuPae、RuP3、RuP4, and CuB1、RuP3、RuP4 three compounds have not been reported. 3. The anti-tumor activities of eight of the paeonol derivatives were determined by MTT assay. The results showed that paeonol derivatives suppress proliferation of K562、HL60 cells in a dose-dependent manner. The inhibiting rate of CuB1 on K562 cells was the highest with IC50 6.94 μg/mL, then IC50 of CuPae, RuP4, RuP3 were 8.58μg/mL ,10.65μg/mL,17.72μg/mL respectively. The inhibiting rate of CuPae on HL60 cells was the highest with IC50 1.96μg/mL, then IC50 of CuB1, RuP4, RuP3 were 2.03μg/mL, 9.31μg/mL, 9.55μg/mL respectively. Which showed CuB1 and CuPae display higher inhibitory activities on proliferation of K562、HL60 cells in a dose-dependent manner, they could be used as research emphasis to further development of this kind compounds. Key words: Paeonol derivatives; Schiff base; Synthesis; Anti-tumor activity 缩略词表 表 1 丹皮酚衍生物的缩写表 Tab 1 Abbreviations of paeonol derivatives 缩写代号 中文及英文全称 结构示意图 N’-[1-(2,4-二羟苯基)亚乙基]苯甲 酰肼 B1 N-[1-(2,4-dihydroxyphenyl)ethylid- ene]benzohydrazide 双（2’.4’-二羟基-α-甲基苄基）-二  HO OH O N NH 亚胺 HO OH HO OH B2 Bi(2’,4’-dihydroxy-α- N N methylbenzylidene) –diimine 双（2’,4’-二羟基-α-甲基苄基）-乙 二亚胺  HO OH  HO OH B3 Bi(2’,4’-dihydroxy-α-methylbenzyl- N N idene)-ethylenediimine 双（2’,4’-二羟基-α-甲基苄基）-1,3- 丙二亚胺  HO OH  HO OH B4 Bi(2’,4’-dihydroxy-α-methylbenzyl- idene)-1,3-trimethylenediimine 5-甲氧基-2-[1-（丙亚胺基）乙基] 苯酚 P1