带蒂筋膜瓣包裹组织工程骨血管化的实验研究-外科学(骨外科学)专业论文.docx

新疆医科大学医学硕士学位论文 新疆医科大学医学硕士学位论文 PAGE PAGE 7 带蒂筋膜瓣包裹组织工程骨血管化的实验研究 研究生：王炜 导师：艾合买提江·玉素甫 摘 要 目的：比较复合人类重组骨形态发生蛋白-2（rhBMP-2）的自固化磷酸钙人工 松质骨和单纯的自固化磷酸钙人工松质骨在比格犬体内被带蒂筋膜瓣包裹后的血管 再生能力。方法：12 只成年比格犬，根据植入材料不同分 2 组进行自身对照，即左 侧的复合 rhBMP-2 的支架材料组（实验组）和右侧的单纯支架材料组（对照组）， 分别包裹于腰背部两侧带蒂深筋膜瓣中，于术后第 2、4、8、16 周各随机选取 3 只 动物后摘取血管化标本，进行大体观察、切片 HE 染色、Masson 染色及血管内皮细 胞Ⅷ因子相关抗原（ⅧRAg）免疫组织化学染色观察比较新血管的生成。结果：在 材料植入后 4 周、8 周和 16 周，两组均发现带蒂筋膜与支架材料接触区出现明显血 管化，并随时间延长，向材料内部延伸；实验组血管相对百分比和ⅧRAg 阳性区比 例在 2、4、8、16 周时均高于对照组。结论：带蒂筋膜瓣促进了支架材料的血管化， 而带蒂筋膜瓣对 rhBMP-2 与支架材料复合体的血管化作用更为明显，并具有一定的 成骨能力。 关键词：组织工程骨；血管化；带蒂筋膜瓣；人类重组骨形成蛋白-2；血管内皮细 胞Ⅷ因子相关抗原 Experimental Study On Vascularization Of Tissue Engineered Bone Wrapped By Pedicled Deep Fasica Flaps Postgraduate: Wang Wei Supervisors: Aihemaitijiang·Yusufu professor Abstract Objective: To compare the vascularization of a complex of scaffold /rhBMP-2 and a single scaffold both implanted in beagle dogs and wrapped by pedicled deep fasica flaps. Methods: Twelve healthy adult beagle dogs were respectively implanted with complex of scaffold /rhBMP-2 in left thigh (experimental sides) and a single scaffold in right thigh (control sides) . The vascularization in each condition was assessed by experiment study (Physical, Masson stain, HE stain, ⅧRAg marked) at time intervals of 2, 4, 8 and 16 weeks after operation. Results: In experimental groups and in control groups, vascularization was found. New vascular invaded in scaffold with following the time. In experimental groups, the amount of vessels and the expression of ⅧRAg were more stronger than there in control group at 2-8 weeks. Conclusion: The pedicled deep fasica flaps have great effect on the vascularization. The effect of the pedicled deep fasica flap is proved to be the better in vascularization and of rhBMP-2/ scaffold. Key word: tissue engineered bone; vascularization; pedicled fasica flap; rhBMP-2; ⅧRag 5 5 中英文缩略词对照表 缩略词 BMP 英文全称 Bone morphogenetic protein 中文全称 骨形态发生蛋白 TEB Tissue engineering bone 组织工程骨 Ang Angiopoie