植物乳杆菌ST-Ⅲ蛋白酶活性的研究-食品科学.PDF

224 《乳业科学与技术》 2009 年第5 期 （总第138 期） 植物乳杆菌ST-Ⅲ蛋白酶活性的研究 * 陈 帅，郭本恒，吴正钧，王荫榆 （光明乳业股份有限公司技术中心，上海 200436） 摘 要：植物乳杆菌ST-Ⅲ（Lb. plantarum ST-III）是一株具有降低血清胆固醇能力的益生菌，其在脱脂 乳中生长缓慢，产酸能力弱。实验选取一株凝乳性较好的植物乳杆菌CZ 2112 作为参照，对植物乳杆菌 ST-Ⅲ蛋白酶活性进行研究。植物乳杆菌ST-Ⅲ蛋白酶活性及蛋白水解能力比植物乳杆菌CZ 2112 低， 而氨肽酶和羧肽酶活性与之差异较少，推测得出植物乳杆菌ST-III 在脱脂乳中不能很好生长和凝乳的 原因是该菌株蛋白酶活性较低，生长初期不能很好利用外源蛋白质营养。在添加鱼蛋白胨等促进生长 后，植物乳杆菌ST-Ⅲ可以将蛋白质、多肽水解成一系列的寡肽或氨基酸，满足菌体生长的氮素营养。 关键词：植物乳杆菌ST-Ⅲ；蛋白酶；氨肽酶；羧肽酶 中图分类号：Q939.98 文献标识码：A 文章编号：1671-5187 （2009）05-0224-04 Study on the Protease Activity of Lactobacillus plantarum ST-III * Chen Shuai, Guo Benheng , Wu Zhengjun, Wang Yinyu （Technology Center of Bright Dairy Food Co., Ltd, Shanghai 200436, China） Abstract: Lactobacillus plantarum ST-Ⅲ，a probiotics with ability to remove cholesterol in vitro and in vivo, demonstrated weak ability to grow in skim milk and to clot milk by acidification. To illustrate the impacts of the proteolytic activity of Lb. plantarum ST-Ⅲ on the growth of itself, the extracellular protease and intracellular peptidase activity of Lb. plantarum ST-Ⅲ were compared with Lb. plantarum CZ 2112, a strain growing well in skim milk supplemented with glucose and clotting milk proteins rapidly. Compared with Lb. plantarum CZ 2112, Lb. plantarum ST - Ⅲ had lower protease activity, whereas there was no obvious difference in the activity of aminopeptidase and carboxypeptidase activi－ ties between the two Lactobacillus strains. Therefore, it was presumed that deficiency inprotease activi－ ty of Lb. plantarum ST-Ⅲ, which was responsible for hydrolyzing large milk proteins into small pep－ tides when the bacteria were cultivated in skim milk, was correlated with the poor ability of Lb. plan－