非小细胞肺癌患者化疗前后外周血sPD-L1的动态变化及CTL联合PD-L1单抗对肺癌细胞的体外研究内科学专业论文.docxVIP

PAGE PAGE VI 非小细胞肺癌患者化疗前后外周血 sPD-L1 的动态变化及 CTL 联合 PD-L1 单抗对肺癌细胞的体外研究 中文摘要 【结果】三种肺癌细胞 H1299，SPC-A1，A549 细胞膜表面表达 PD-L1 呈高，中， 低表达。CTL 联合抗 PD-L1 单抗组较 CTL 组可进一步增强 T 淋巴细胞增殖。肺癌细 胞株 H1299，A549 的迁移侵袭能力优于 SPC-A1。与肺癌细胞共培养 12h，24h，48h 后 CTL 联合抗 PD-L1 单抗组较 CTL 组更显著的抑制肿瘤细胞的迁移。 【结论】CTL 联合 PD-L1 单抗能增强 T 淋巴细胞抗肿瘤免疫效应，在体外有效 抑制肿瘤细胞的生长和侵袭。 综上所述，本课题研究得出以下结果：（1）运用人 sPD-L1 ELISA 试剂盒，研 究发现 NSCLC 患者外周血清中 sPD-L1 的表达异常增高，且其高表达与肺癌的临床 分期、淋巴结转移和疗效有关。（2）通过 PD-L1 阻断型单抗作用于 PD-1/PD-L1 信 号通路，T 淋巴细胞增殖的能力可以部分恢复，CTL 联合 PD-L1 单抗能增强 T 淋巴 细胞抗肿瘤免疫效应，在体外有效抑制肿瘤细胞的生长和侵袭。 【关键词】程序性死亡配体-1；肺癌；协同刺激分子 作 者：王晓景 指导教师：施敏骅 英文摘要 非小细胞肺癌患者化疗前后外周血 sPD-L1 的动态变化及 CTL 联合 PD-L1 单抗对肺癌细胞的体外研究 Dynamic changes of soluble PD-L1 before and after chemotherapy in patients with lung cancer and the effect of CTL with PD-L1 monoclonal antibody in inhibiting lung cancer cell in vitro Abstract Lung cancer was one of the most common malignancies, showing a trend of rapid rise on the incidence and mortality rates, and it ranked the leading cause of death of various types of cancer. Escape mechanism of tumor immune was the research focus in recent years. Costimulatory molecules participated in immune regulation, and it’s abnormal expression in immune escape of tumor cells played a very important role. Among the new members of CD28/B7 costimulatory superfamily, PD-1/PD-L1 could mediate negative costimulatory signals which inhibit the activation and proliferation of T and B lymphocyte. The inhibitory pathway involved in immune regulation and played important significance in the study of tumor immunity. Costimulatory molecules had two forms, soluble and membrane types. Many clinical studies showed that the expression level of the body soluble costimulatory molecules possed diseases’ diagnostic and predictive potential. At present, because of the lack of effective detection methods of soluble PD-L1 (sPD-L1), the study of the report was still rare at home and abroad. It was still unknown remains to be clarified if sPD-L1 expression levels in non-s