磁共振氢频谱检测乳酸的脉冲序列优化及其在脑疾病诊断价值的研究-医学影像学专业论文.docx

汕大医学院 汕大医学院硕士学位论文 万方数据 万方数据 Study on optimization of pulse sequences for lactate detection and its diagnostic value in brain diseases using 1H MR spectroscopy Shantou University Medical College Postgraduate: Rao Haibing Supervisor: Professor Wu Renhua 【Abstract】 Objective Lactic acid is one of metabolites from the glycolysis. Brain anoxia and ischemia will cause accumulation of lactate. Non-invasive detection of lactate using MR spectroscopy has played an important role in diagnosis and treatments of diseases due to anoxia and ischemia. However, peaks related to lactate are usually difficult to be detected in most of cases, which might be an obstacle for the analysis of illness. The purpose of present study was to obtain optimal detection and quantitative analysis of lactate by changing a series of parameters used in the sequences. Moreover, we do more to get the most optimization sequences and carry out more clinical trials to validate the sequences, therefore we can solve the technical problem in detection and quantitative analysis of lactate using MRS. Methods The studies were performed on both phantom and human subjects. The phantom experiment was carried out in a glass model containing lactate. The MR scanner is a 1.5T system (Signa MR/i, GE). The spectra were obtained by using both point resolved spectroscopy (PRESS) and stimulated echo acquisition (STEAM) sequences. In order to obtain the optimal TE and TR values of the metabolites in the brain and external standard solution, MRS data were collected with different TE values at a constant TR and different TR values at a constant TE. The spectra were processed using a GE software. The peak heights were measured and the curves of the peak heights versus the TE were plotted using Microsoft Excel 3 program. Moreover, the optimal sequence combining with other sequences, which including T1WI，T2WI，FLAIR and DWI, were used to acquire proton MRI data for 17 patients with acute cerebral infarction and 20 healthy volunte