磁性纳米二氧化硅的研发-生物工程专业论文.docx

东华大学硕士毕业论文 东华大学硕士毕业论文 万方数据 万方数据 （2）为了提高磁性复合粒子的均一性，本文用油酸（OA）修 饰了 Fe3O4 磁性纳米粒子，利用偏硅酸钠水解法合成了 Fe3O4@SiO2 磁性复合粒子，最后得到的磁性复合粒子相对于以水溶性 Fe3O4 合成 的磁性复合粒子均一性明显改善。由于 Fe3O4/OA 粒子在醇水中分散 性较差，因此，本文以甲苯作共溶剂，将 Fe3O4/OA 粒子分散在醇水 中，利用偏硅酸钠水解法法实现 SiO2 的包覆。因为 Fe3O4/OA 粒子的 均一性好于 Fe3O4，因而得到的磁性复合粒子的分散性也要明显好于 以水溶性 Fe3O4 合成的磁性复合粒子。制备的磁性复合粒子粒径约为 20nm。 （3）根据小试实验所得的最佳反应条件，用 5L 反应釜进行放大 试验。对产物进行 TEM、XRD 及 FTIR 测试。实验结果表明：放大 试验产品颗粒粒径范围与小试产品相同。 （4）本文对合成的 Fe3O4@SiO2 磁性复合粒子上偶联 KH560 进 行了氨基修饰。Fe3O4@SiO2 与蛋白质分子的结合，达到蛋白质分离 的目的。经计算 1.0mg 磁性纳米粒子结合的牛血清蛋白的质量约为 17.1×10-6g。本文同时探索了 Fe3O4@SiO2 磁性复合粒子对黄酮类化合 物的选择性吸附研究。结果表明 Fe3O4@SiO2 磁性复合粒子对黄酮类 化合物有明显的吸附作用，具有良好的应用前景。 研究磁性纳米二氧化硅低成本，高产量的合成技术，有利于磁性 二氧化硅纳米材料生产成本的显著降低，从而促进磁性纳米二氧化硅 产业化的发展。 关键词：磁性复合粒子，Fe3O4，Fe3O4@SiO2 磁性复合粒子 II RESEARCH AND DEVELOPMENT OF MAGNETIC NANO-SILICA ABSTRACT As a new functional material, the study of magnetic composite particles has developed quickly in recent years. It’s applications in immunoassay lead to a new idea for bio-separation and detection. The development of magnetic particles with functional unites was described in this paper, and displayed it’s application in cell separation, fixation of enzyme, target presenting, protein purification, nucleic acid separation and clinical diagnosis. The aim of this thesis is to prepare Fe3O4@SiO2 composite functional particles with simple operation and high quantity. Their chemical and physical characteristics were analysed comprehensively. In this paper, chemical co-precipitation method was used to prepare the magnetic particles with hydriphilicity. The Fe3O4@SiO2 composite particles are obtained through sodium silicate of hydrolyzation, and SiO2 was produced on the surface of Fe3O4 particles. The composite particles are characterized by a series of techniques including TEM, XRD, FTIR and VSM. Some factors that would influence the result were discussed in particular, such as interaction temperature, time, concentration of regents, pH, and etc. The TEM images showed that the nanoparticles were favorab