《恩施富硒植物硒蛋白抗氧化性研究》-毕业论文（设计）.doc

恩施碎米荠叶片含硒蛋白的体外抗氧化研究 摘 要 硒在动物和人体生命活动中具有重要作用，兼具营养、致毒、疾病治疗等多种生物学功能。以恩施高硒区（恩施双河乡鱼塘坝）发现的一种富硒能力极强的十字花科碎米荠属（Cardamine L）植物碎米荠（Cardamine sp.）为材料，建立了一个基于盐析和凝胶分离的蛋白质纯化流程；模拟机体产生超氧阴离子的体系研究了纯化的含硒蛋白抗自由基的活性；以昆明小鼠为研究对象，探讨了纯化含硒蛋白的体外抗氧化活性。研究显示：对恩施碎米荠叶片可溶性蛋白采用35%-55%饱和度硫酸铵沉淀，得到的含硒蛋白具有清除自由基和抗脂质过氧化的能力，含硒蛋白溶液的硒浓度为0.95 μg/mL时，对羟自由基和超氧阴离子的清除率超过50%， 含硒5.96 μg/mL的蛋白溶液对MDA生成的抑制率达到62.27%；在体外，对小鼠线粒体和肝脏MDA的生成具有明显的抑制作用，硒质量浓度为5.96 μg/mL的含硒蛋白溶液可以保护红细胞免受过氧化损伤。结果表明叶片中的含硒蛋白具有稳定的抗氧化活性。 关键词：碎米荠；硒；含硒蛋白；抗氧化 Antioxidant Research In vitro of the Selenium protein in the Cardamine sp. Leaves from Enshi Abstract selenium plays an important role in both nutrition, toxigenicity, disease treatment and so many kinds of biological function in animal and human life activity.The raw materials Cardamine is a plant of cruciferous Cardamine genera (Cardamine L) plants (Cardamine sp.) was found in Enshi high-selenium area (Yu tang ba in enshi double river township ) which has a strong se-enriched ability .established a separate based on salting-out and gel protein purification process， simulated a system of superoxide anion produced in body ,researched the anti-free radical activity of the selenium protein .To kunming mice as the research object, and discusseed the purification containing selenium protein in vitro antioxidant activity. Research showed that:the Soluble selenium protein in the Enshi Cardamine leaves has the ability of clearing the Supressing lipid peroxidation and Free radicals after it was precipitated by the Ammonium sulfate solution(35% - 55% ) ,when the concentration of the Soluble selenium protein is 0.95 μg/mL ,the rate of the cleared Hydroxyl radicals and superoxide anion is above 50%,the generated inhibition rate to the MDA reached 62.27% when Soluble selenium concentration is 5.96 μg/mL,under this rate,the red blood cells damage can be prevented from peroxidation at the same time.The Soluble selenium protein has a obvious inhibition to the mitochondria and liver MDA generation In vit