降解胆固醇马红球菌F21的分离鉴定及其特性研究-河北工业科技.PDF

25 1 河 北 工 业 科 技 V ol . 25, No . 1 200 8 1 H ebei J our nal of Indu str ial Science and T echno log y J an . 2008 1008- 1534( 2008) 0 1-0005- 05 F21- 1 1 2 1 1 , , , ( 1. 中国农业大学食品科学与营养工程学院, 北京 100083; 2. 河北科技大学生物科学与工程学 院, 河北石家庄 050018) 利用以胆固醇为 一碳源的选择性培养基, 从豚鹿粪便样品中分离出1 株降解胆固醇良好 的菌株经生理生化鉴定和 16S rDN A 基因序列分析, 该菌株为马红球菌 ( Rhodoc oc cus equi ) 该菌株降解胆固醇的最佳碳源为胆固醇, 最佳氮源为蛋白胨硝酸铵复合氮; 最佳培养条件 温度为 32 , 初始p H 值为8. 0, 胆固醇质量浓度为1. 50 g/ L在该培养条件下培养24 h , 胆固醇的降 解率可达到71. 95% 马红球菌; 降解胆固醇; 筛选; 鉴定 T S201. 3 A Isolat ion, identif icat ion of a new R hodococcus eq ui F2 1- 1 w it h cholest erol degrading act ivit y and stu dy on it s charact erist ic 1 2 1 1 ZH A N G Bin , H A N Jun- hu a , JIA N G Dan- dan , N IU T ian- g ui ( 1. Colleg e o f Fo od Science and N utritio nal Eng ineering , China A g ricult ur e U niver sity, Beijing 1000 83, China; 2 . Co lleg e o f Bio lo gical Science and Engineer ing , H ebei U niver sity o f Science and T echnolo gy , Shij iazhu ang H ebei 050018, China) Abstract: A new st rain w hich is able to degr ade cho lestero l w as iso lated fr om Ho g Deer f eces by u sing selectiv e m edium . T he select ive str ain can use cholester ol as the so le carbon so ur ce. A fter the analysis of phy siolog ica-l bio chem istr y ch aracterist ic and the 16S r DN A g ene sequ ence, the st rain w as identif ied as R hod ococcus equi. T he o ptimum carbon so ur ce is cholester ol , and the optimum nitro gen source is p epto ne. T he optimum f erm entatio n condit i