中文题目反复接触七氟醚会损害老年雄性大鼠的学习能力和记忆英文.PDFVIP

中文题目 反复接触七氟醚会损害老年雄性大鼠的学习能力和记忆 英文题目 Repeated exposure to sevoflurane impairs the learning and memory of older male rats 译 者：李华宇 背景与目的：危重病患者常需要反复手术干预，因此探讨反复接触麻醉药物对 学习和记忆的影响就显得非常重要。七氟醚是一种广泛使用的吸入麻醉剂，对 神经系统几乎没有副作用，并能使意识迅速恢复。但七氟醚暴露的长期影响和 重复七氟醚暴露后对认知功能的影响却少有文献报道，这与现有的研究相矛盾。 方法：在本研究中，Morris 水迷宫试验被用来研究单次（4 小时）或重复（每 天 2 小时连续 5 天）暴露于 1.5％或 2.5％七氟醚后对老年（16-18 月龄）雄性 大鼠学习能力和记忆的长期影响。测试是从最后一次暴露后 1 天到 4 周内进行。 在海马中，通过对脑源性神经营养因子（BDNF ），NF-κBmRNA 和细胞凋亡 率进行检验，以确定在单次或重复接触七氟醚后对认知功能和记忆的影响是否 与细胞生化的改变有关。 结果：反复接触 2.5％七氟醚降低了老年雄性大鼠海马的 BDNF 蛋白水平，提 高了海马的 NF-κBmRNA 水平，增加了锥体细胞凋亡率。单次暴露于 2.5％七 氟醚以及反复接触 1.5％或 2.5％七氟醚显着损害了老年雄性大鼠的学习能力和 记忆。 结论与讨论：反复接触七氟醚会损害老年雄性大鼠的学习能力和记忆，这种损 伤伴随着海马内与认知功能相关的生化改变。 原始文献摘要 Guo S1, Liu L2, Wang C2, Jiang Q2, Dong Y2, Tian Y3. Repeated exposure to sevoflurane impairs the learning and memory of older male rats. Life Sci. 2017 Nov 16. pii: S0024-3205(17)30602-1. doi: 10.1016/j.lfs.2017.11.025. [Epub ahead of print] Abstract Aims: Critically ill old patients sometimes require repeated surgical interventions, and thus it is important to determine the influence of repeated exposure to anesthetics on learning and memory. Sevoflurane, a widely used inhalation anesthetic, has few neurological adverse effects and offers a rapid return to consciousness. But the long-term influence of sevoflurane exposure and the effect of repeated sevoflurane exposure on cognition have rarely been reported, and available studies are contradictory. Materials and methods: In the present study, the Morris water maze test was employed to investigate the longterm influence of single (4 h) or repeated (2 h daily for 5 consecutive days) exposure to 1.5% or 2.5% sevoflurane on the learning ability and memory of old (16–18 months old) male rats. Testing was performed from 1 day to 4 weeks after the last exposure. In the hippocampus, brain derived neurotrophic factor (BDNF), NF-κB mRNA, and apoptosis rate were also examined to determine whether cellular bioche