网站大量收购独家精品文档,联系QQ:2885784924

地西他滨联合改良CAG及半相合外周血淋巴细胞回输治疗老年AML的临床及机制分析-内科学(血液病学)专业论文.docx

地西他滨联合改良CAG及半相合外周血淋巴细胞回输治疗老年AML的临床及机制分析-内科学(血液病学)专业论文.docx

  1. 1、本文档共65页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
地西他滨联合改良CAG及半相合外周血淋巴细胞回输治疗老年AML的临床及机制分析-内科学(血液病学)专业论文

解放 解放军医学院硕士学位论文 PAGE PAGE 10 促进地西他滨的诱导分化的能力;地西他滨能上调 G-CSF 的受体 CSF3R 的 mRNA 水平的表达,但不能上调其蛋白质水平的表达,CSF3R 的 5’UTR 为低甲基化状 态。 结论:DAC 联合改良 CAG 及半相合外周血淋巴细胞回输方案作为老年 AML 患者 的诱导治疗能获得较高的完全缓解率,能明显缩短血细胞减少的持续时间,同时 不会发生 GVHD,是治疗老年 AML 患者的一种安全的、有效的诱导治疗方法。 G-CSF 促进了地西他滨诱导髓系白血病细胞株分化的能力,可能是临床方案疗效 好的原因之一。 关键词:地西他滨、粒细胞集落刺激因子、老年急性髓系白血病、半相合外周血 淋巴细胞回输 Abstract Objective: The elderly patients with acute myeloid leukemia (AML), accounting for about 55% of all AML patients, often have a high chemotherapy-related mortality and a low survival. An effective and less toxic induction therapy is required for the treatment of elderly patients with AML. We conducted a prospective single-arm open-label clinical trial of decitabine and modified CAG (cytarabine, aclacinomycin and G-CSF) chemotherapy plus haplotype identical donor lymphocyte infusion regimen as induction chemotherapy in elderly patients with AML. The efficacy and safety of the regimen were evaluated. The effects of decitabine and G-CSF in combination in human myeloid leukemia cell lines were explored . Methods: A total of 20 elderly patients with newly diagnosed AML from April, 2012 to March, 2013 were enrolled. Clinical characteristics, response to the regimen, treatment-related adverse events and graft-versus-host disease (GVHD) were analyzed. Four myeloid leukemia cell lines (Kasumi-1、SKNO-1、THP-1 and U937) were treated with no drugs, G-CSF alone, decitabine alone, decitabine and G-CSF, respectively. After two days of treatments, cell apoptosis and differentiation of the leukemia cell lines were quantified by flow cytometry. Cell viability was measured at 24h, 48h and 72h after treatments by Cell Counting Kit-8. Colony formation assay was conducted in Kasumi-1 and THP-1 cells. Real-time quantitative RT-PCR and flow cytometry were used to observe the changes of gene CSF3R (G-CSF receptor) in the mRNA and protein levels. Bisulfite sequencing was performed to study the methylation status in the 5’UTR of CSF3R. Results: The average age

您可能关注的文档

文档评论(0)

peili2018 + 关注
实名认证
内容提供者

该用户很懒,什么也没介绍

1亿VIP精品文档

相关文档