富油微藻中试产业化研究-生物化学与分子生物学专业论文.docx

内蒙古 内蒙古科技大学硕士学位论文 PAGE PAGE IV Abstract With the need to reduce carbon emissions, and the dwindling reserves of crude oil, green, clean, renewable biodiesel is an attractive source of energy. Microalgae have gained a great deal of attention as a potential biodiesel raw material because of their high growth rates and ability to accumulate oil, bind carbon dioxide, and remove contaminants from wastewater.Nowadays, the cost of microalgae biodiesel was still high, needing for media optimization, large-scale cultivation of microalgae and use the wastewater culture microalgae to reduce it cost. And cut down the biodiesel cost. The purpose of this thesis is screening the high-lipid microalgae strains with high biomass from Wuliangsuhai, Tianjin port of BoHai bay. Discover four oil-rich strains, two freshwater algae: Scenedesmus quadricanda and Scenedesmus obliquus, two sea algae: marine diatom and Nannochloropsis. Then adjust the best of biomass accumulation, culture condition, scale cultivation, and using the wastewater make it grow to reduce cost. The major results are shown as follows: Discover four algal strains：Scenedesmus quadricanda, Scenedesmus obliquus, marine diatom and Nannochloropsis. The initial biomass and lipid content were 0.93 g/L (17.42%), 1.14 g/L (15.83%), 0.77 g/L (21.71%), 0.77 g/L (23.31%). The fatty acids of the four microalgae, with a majority as oleic acids and palmitic acids, and contain Linolenic, Linoleic, EPA, DHA. If dissociate those polyunsaturated fatty acids from extracted oil, can cut the cost. In order to enhance microalgae biomass and oil content, optimizing the nutrients that N, P, Fe, C and Vit B1 and using a single factor experiment and L9(34) orthogonal design method. The results showed, the optimal concentrations of four kinds of nutrients was： Scenedesmus quadricanda: the sodium nitrate 1.5 g/L, the monopotassium phosphate 0.4 g/L, the chelated iron ions 0.0002 mol/L and Sodium bicarbonate 7.5 g/L, reaching the maximum growth