复方雪莲胶囊的药效学分析-药理学专业论文.docx

复方雪莲胶囊的药效学研究 复方雪莲胶囊的药效学研究 II II Abstract This research is to pharmacodynamic re-evaluate anti-inflammatory and analgesic effects of Compound Xuelian Capsule and establish cattle Collagen-induced arthritis(CIA)，to discuss further the action mode of Compound Xuelian Capsule cure rheumatoid arthritis, to provide experimental basis for clincical application. Objective: (1) To re-evaluate anti-inflammatory and analgesic effects of Compound Xuelian Capsule. (2) Observe therapeutic action of collagen-induced arthritis rats，and investigate the mechanisms of therapeutic effect by survey serum factor: TNF-α and PGE2 of CIA rats. Method:1.Anti-inflammatory action test: The anti-inflammatory effects were investigated by ear edema of mice induced by xylene, the permeability of celiac blood capillary and carrageen induced white blood cells (WBC) movement in rat chest; 2. The Acetic acid writhing was used to observe analgesic effect; 3. adjuvant-induced arthritis in rats; 4. The therapeutic action of Collagen-induced arthritis rats: The therapeutic effect was investigated by measure and observe the effect of Compound Xuelian Capsule on the ankle swelling of CIA rats. Take the synovial tissue, make biopsy and observe the synovial histopathology of rats; 5. The inflation of the serum factor: The phlogistic cell factor TNF-α, PGE2 was detected by enzyme linked immunosorbent assay (ELISA) to investigate some of the mechanisms. Result: (1) Compound Xuelian Capsule could suppress mice ear swelling by xylene. (2) it could inhibit markedly the increase in abdomen capillary permeability in mice induced by acetic acid, lower the permeability of celiac blood capillary. (3) It could markedly reduce the number of WBC in the thorax extravasate of rats induced by carrageenan. 2. Compound Xuelian Capsule could decrease the number of writhes of mice caused by acetic acid. 3. Compound Xuelian Capsule could significantly inhibit the primary and secondary footpad swelling of rats ind