低硒对发育期大鼠脑中CREB及核基质蛋白影响的研究-法医学专业论文.docx

万方数据 万方数据 The Effects of Selenium Deficiency Diet on CREB and Nuclear Matrix Proteins in Brain of Developing Rat Postgraduate: Geng Yiqun Supervisor: Prof.Xu Xiaohu Prof.Su Min Abstract Object To observe the changes of lipid peroxide in the cortex and hippocampus of developing rat induced by selenium deficiency diet and study the neuron function by the content and activity detection of cAMP Response Element-Binding Protein (CREB) in cortex and hippocampus. To study the alteration of NMPs components. Material and method Groups of healthy adult SD rats with equal male and female at random were fed with selenium deficiency and selenium adequate food. The third generation at 21 days of age were killed and the cortex and hippocampus were dissected on ice rapidly. The contents of MDA were assayed by 2-thiobarbituric acid (TBA) test. The expression of CREB and p-CREB were examined by western blot in the extracted total protein from cortex and hippocampus. The expression and the location of CREB and p-CREB were further explored in different kind of neuron in the same region of brain by paraffin brain section immunohistochemistry. The expression of p-CREB was also detected from the extracted NMPs of rat brain by western blot. The component of the NMPs between two groups were analysed and compared by High Performance Capillary Electrophorisis(HPCE). Result The content of MDA in the cortex and hippocampus of selenium deficiency group was much higher than that of the control group(control cortex MDA＝0.31± 0.13, selenium deficiency cortex MDA=0.66±0.10,P0.05;control hippocampus MDA=0.42±0.11 ， selenium deficiency hippocampus MDA=0.89±0.066);The detection of CREB and p-CREB from the total protein showed that the expressions of selenium deficiency group were lower than that of control group. The grey degree of CREB positive cells in selenium deficiency cortex and hippocampus that were examined by immunohistochemistry increased (control c