当归挥发油对兔十二指肠平滑肌的作用及有效组分筛选研究-中药学专业论文.docx

当归挥发油对兔十二指肠平滑肌的作用及有效组分筛选研究 当归挥发油对兔十二指肠平滑肌的作用及有效组分筛选研究 PAGE PAGE 4 According to AN by the oil mass ratio of each component, to the orthogonal design and analysis of the three active components effects are different of the rabbit duodenal smooth muscle showed that A3 components can significantly inhabit rabbit duodenal smooth muscle main factors, and the role of the total oil AN rather, A1、A2、A3 components had no significant effect interaction on the rabbits smooth muscle. Conclusions AN obviously inhibited contraction on isolated rabbit duodenal smooth muscle by Ach or not, the effect concerned with inhibiting the Receptor-Operating Calcium Channels (ROCC) or Voltage-Dependent Calcium Channels(VDCC) 、 and extracellular Ca2+ influx and intracellular Ca2 + release processes on the rabbit duodenum smooth muscle. A1、A2、A3 significantly inhibited on isolated rabbit duodenal smooth muscle, and its effect was no significant difference; But A3 is the highest levels, A1 and A2 is liittle in AN, A3 is to play the major factors inhibit in AN on the isolated rabbit duodenal smooth muscle; A1、 A2、A3 were no interaction between the components on the rabbit duodenal smooth muscle. KEY WORDS Angelica Naphtha; Duodenal smooth muscle;Mechanism of action;Effective components 缩 略 词 表 缩写 英文全称 中文全称 AN Angelica naphtha 当归挥发油 A1 Angelica naphtha Ⅰ 当归挥发油酸性部位 A2 Angelica naphtha Ⅱ 当归挥发油酚性部位 A3 Angelica naphtha Ⅲ 当归挥发油中性非酚性部位 Ach Acetylcholine 乙酰胆碱 Atr Atropine Sulfate 硫酸阿托品 Ver Verapamil Hydrochloride 盐酸维拉帕米 GC-MS Gas chromatography- mass spectrography 气相色谱-质谱 LIG Ligustilide 藁本内酯 NMR Nuclear magnetic resonance 核磁共振 SCF-CO2 Supercritical CO2 fluid extraction 超临界 CO2 萃取 SD Steam distillation 水蒸气蒸馏 VDCC Voltage-depended calcium channel 电压依赖性钙通道 RO