德国镜鲤与黑龙江野鲤杂交组合的亲子鉴定及鲤鱼免疫功能基因SNP位点分析-水产养殖专业论文.docx

上海 上海海洋大学硕士学位论文 II II TLR9基因 TLR9基因在 292 个个体中共检测出 37 个变异位点，其中 20 个变异位点引起 氨基酸的变化。c.988CT、c.1237TC、c.1700TA、c.1770AG、c.2278TC、 c.2307TC、c.2377AG、c.2547AT、c.2580TA、c.2646TC 在 8 个鲤鱼群体分 布频率最高，其中 c.2580TA、c.2646TC 在 8 个鲤鱼群体中均有分布。 MyD88a 和 MyD88b 基因 在 MyD88a 基因中共检测出 6 个变异位点，均位于第 4 内含子区域。在 MyD88b 基因共检测出 9 个变异位点，其中 1 个为非同义突变。 关键词：鲤鱼，微卫星多态性，亲子鉴定，遗传结构，鲤鱼免疫功能基因，SNP PAGE PAGE IV Parentage Identification of Heilongjiang wild carp and the German mirror carp cross combinations on Microsatellite DNA Markers and Carp immune function gene SNP research ABSTRACT A set of 8 microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp♀×German mirror carp♂, and inverse cross group of the German mirror carp♀×Heilongjiang carp♂. The results showed that: (1) observed heterozygosity values of parents and offsprings were 0.607 1, 0.714 3, 0.681 8 and 0.708 3, 0.538 6, 0.743 8, and polymorphism information content were 0.442 1, 0.281 3, 0.553 5 and 0.673 5, 0.375 9, 0.624 1 in two groups, indicating that all 8 microsatellite loci showed polymorphism. (2) In the obverse cross group, the accumulative exclusion rate using 8 microsatellite loci was 99.92% and the appraisal ability was 98.00% while confidence was 95%. In the inverse cross group, the accumulative exclusion rate was 99.99% and the appraisal ability was 99.00%. (3) about relation between microsatellite markers and appraisal ability, we compared the accumulative exclusion rates and the appraisal ability using 3, 4, 5, 6, 7, 8 microsatellite markers. 6 and 8 high polymorphism markers were successfully applied in paternity deter mination of two cross groups. The study of carp immune function genes showed that: (1) four of the 11 carp immune function genes had the SNP. TLR2, TLR3a TLR3b, TLR7a, TLR7b, I type of IFN, and TRAF6 had no SNPs after SSCP detection in 292 individuals of 8 carp populations, but IL-1β, TLR9, MyD88a, MyD88b gene polymorphism were higher. (2) about IL-1β gene, 13 variabl