肥胖及糖尿病大鼠肝脏组织中SOCS-3mRNA和OB-RbmRNA的表达相关性内分泌与代谢病专业论文.docxVIP

青海大学硕士学位论文英文摘要 青海大学硕士学位论文 英文摘要 II II Abstract Objective:To investigate the expression of SOCS-3 mRNA and OB-Rb mRNA in liver tissue of obesity and diabetic rats for understand the mechanism of leptin resistance . Methods: One hundred male Wistar rats weighted (150±10)g were accommodated to feed with normal chow for a week and then divided into three groups randomly:the normal control group(NC group,n=15) fed with normal chow,the diet-induced obese group(DIO group,n=35) fed with high-fat diet,the diabetic group(DM group,n=50) were fed with high-fat diet for 4 weeks and then injected strptozotocin(STZ) into abdominal cavity to induce to diabetes model group,the normal control group were injected .All rats were fed with relative fodders for 28weeks. The serum level of leptin was examined by ELISA method. Expressions of SOCS-3 mRNA and OB-Rb mRNA in liver tissue of the 3 groups were investigated by RT-PCR. Results: Compared with control group,the expression level of SOCS-3 mRNA were higher (P0.05),but OB-Rb mRNA was lower (P0.05) in obesity and diabetic rats. There are negative correlation of the expression level between OB-Rb mRNA and Socs-3 mRNA (r=-0.596,P0.05).The regression analysis indicate that the expression level of OB-Rb mRNA was influenced by the expression level of SOCS-3 mRNA and the body weight; the expression level of SOCS-3 mRNA was influenced by the blood glucose ,the serum level of leptin and the expression level of OB-Rb mRNA. Conclusion: The higher serum level of leptin may be increaded the expression of SOCS-3 mRNA and caused the inhibition of expression of OB-Rb mRNA in liver tissue. Key words:Socs-3 mRNA, OB-Rb mRNA, Diabetes, Obesity 青海大学硕士学位论文目录 青海大学硕士学位论文 目录 PAGE PAGE III 目录 中文摘要I HYPERLINK \l _bookmark0 英文摘要II HYPERLINK \l _bookmark1 主要符号对照表IV HYPERLINK \l _bookmark2 第一章 前 言1 HYPERLINK \l _bookmark3 第二章 材料与方法2 HYPERLINK \l _bookmark4 第三章 结 果5 HYPERLINK \l _bookmark5 第四章 讨 论10 HYPERLINK \l _bookmark6 第五章 结论13 HYPERLINK \