蜂毒素对大鼠膀胱癌P16基因表达作用的研究-外科学专业论文.docxVIP

三峡大学硕士学位论文 三 峡 大 学 硕 士 学 位 论 文 PAGE IV万方数据 PAGE IV 万方数据 Abstract Objective: To investigate the effect of melittin on the P16 expression in rat bladder cancer and its probable mechanism involved. Methods: The rat model of bladder cancer in situ，bladder perfusion in rats treated With the MNU (N - methyl phosphite nitrourea) solution 0.5ml every two weeks，observed the tumor after perfused 4 times. After making a successful model, bladder perfusion the rats with different concentrations of melittin (8μg/mL, 16μg/mL) every three days, observed the animals survival rate, calculated the tumor inhibition rate, RT-PCR detection of melittin on rat bladder cancer P16 expression of mRNA. Western blotting analysis of melittin on bladder cancer P16 protein expression. Results: Using MNU solution on rat bladder perfusion after 4 weeks, through the autopsy of rat bladder MNU,find the MNU can induce the occurrence of bladder cancer in rats. Pathological diagnosis of invasive urothelial carcinoma, it can be observed in the section of diffuse distribution or arranged in nests of substantive, focal submucous infiltration, muscle layer, fission as visible. Using 8μg/mL, 16μg/mL melittin intravesical model rats, the rats survival rate was 64.15%, the tumor inhibition rate was 24.03%. With 8μg/mL, 16μg/mL melittin the model rats after intravesical instillation, RT-PCR detection of P16 mRNA expression increased gradually, and in a dose-correlation manner. With 8μg/mL, 16μg/mL bee toxin intravesical mouse model, the amount of protein expression by Western blotting P16 gradually increased in a dose-correlation manner. Conclusion: MNU-induced tumor model is simple, less medication, a short time into the tumor, tumor formation rate, and organ-specific characteristics. Melittin can increase the amount of P16 gene expression, and presents a dose-correlation manner. In the development of bladder cancer in rats P16 gene may be one of the important molecular mechanisms. Key words: MNU