研究用肿细胞系的质量控制和要求.pptVIP

研究用肿瘤细胞系的质量控制和要求 中国医学科学院基础医学研究所 刘玉琴 Some Facts about the use of cultured cells-- Nobel prize 2001，Cell cycle 2002, apoptosis 2006, RNA Interference 2007, homologous recombination and gene targeting 2008,HPV—cervical ca, HIV--aids 2009, telomere telomerase 现代生命科学研究中培养细胞的使用越来越广泛 1969~2004：cell use increasing steadily Papers use cells increase 2~2.5X biology, pharmacology and medicine---- tumor research 不可少的研究材料、对象、工具 Use of contaminated or misidentified cells increase 10X Common problems microbiological contamination with particular respect to mycoplasma infections (incidence of ca. 25%)