Bromodomain-like折叠类型模板的设计.pdfVIP

第42卷 第10期 北 京 工 业 大 学 学 报 Vol.42 No.10 2016年 10月 JOURNAL OF BEIJING UNIVERSITY OFTECHNOLOGY Oct. 2016 Bromodomain鄄like折叠类型模板的设计 李晓琴,张春城 (北京工业大学生命科学与生物工程学院,北京摇 100124) 摘摇 要:针对折叠类型分类中所选天然模板普适性不足的问题,提出了Bromodomain鄄like折叠类型模板的设计方 法. 选SCOPe Astral2郾03序列相似度小于40%并且分辨率高于0郾25nm 的52个可用Bromodomain鄄like折叠样本, 基于多结构比对结果及数据分析,建立了折叠类型家族模板的设计方法. 利用系统聚类方法构建了家族模板的系 统聚类图,提出了蛋白质折叠类型模板的设计方法,并用于该折叠类型的模板设计. 结果表明:设计模板具有普适 性,可用于蛋白质折叠类型分类. 关键词:折叠类型分类;模板设计;结构比对;系统聚类 中图分类号:O641 文献标志码:A 文章编号:0254-0037(2016)10-1572-09 doi:10.11936/ bjutxb2015100078 Design of a Bromodomain鄄like Folding Type Template LI Xiaoqin,ZHANG Chuncheng (College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China) Abstract:For theproblemthattheuniversal shortageof naturaltemplateforfoldingtypeclassification,a design method of the Bromodomain鄄like folding type template was presented. 52 Bromodomain鄄like folding type samples whose sequence similarity is less than 40% were chosen and the resolution was higher than 0郾25 nm in the database of the SCOPe of astral 2郾03. Based on the results of multiple structure alignment and data analysis, the design method of the folding type family template was established. The clustering graph of family templatewasconstructed using the system clustering method, and the design of the template of thefolding typewas completed. Results show that the design templates have universality,and the templates can be used for protein folding type classification. Key words:folding type classification;template design;structure comparison;system clustering 摇 摇 蛋白质的结构能够提供蛋白质的很多信息,有 列相似度的限制,从头