化浊解毒疏肝方对抑郁模型大鼠行为学及 神经肽Y的影响-中西医结合临床专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要 ··········································································· 1 英文摘要 ··········································································· 4 英文缩写 ··········································································· 7 研究论文 化浊解毒疏肝方对抑郁模型大鼠行为学及神经肽 Y 的影响 前言 8 材料与方法 ································································· 9 附表 ··········································································· 17 附图 ··········································································· 21 讨论 ··········································································· 27 小结 ··········································································· 33 参考文献 ···································································· 34 综述 抑郁症的中西医研究进展················································· 36 致谢 ·················································································· 46 个人简历 ············································································ 47 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 化浊解毒疏肝方对抑郁模型大鼠行为学及 海马神经肽 Y 的影响 摘 要 目的： 通 过 建 立 孤 养 和 慢 性 温 和 不 可 预 见 性 应 激 刺 激 （ Chronic unpredictable mild stress,CUMS）抑郁模型，观察化浊解毒疏肝方对慢性 应激抑郁模型大鼠情感行为学和海马 CA3 区神经肽 Y 含量的影响，探讨 中医中药干预治疗抑郁症的可能机制，为进一步开发化浊解毒疏肝方的抗 抑郁疗效提供实验基础。 方法：将 60 只健康清洁级 Wister 雄性大鼠随机分为 6 组，分别为： 正常对照组、模型组、氟西汀组、柴胡疏肝散组、化浊解毒疏肝方高剂量 组和低剂量组，每组 10 只，正常对照组每笼 10 只，给予正常饮食及饮水， 其余各组单笼饲养每笼 1 只并采用以下 8 种应激刺激方法，分别为：禁食、 禁水、昼夜颠倒、夹尾、水平震荡、潮湿垫料、冰水游泳、热刺激，每天 随机安排一种刺激，平均每种刺激使用 2-3 次，刺激方式不可预见，持续 刺激共 21 天来制备慢性温和不可预见性大鼠抑郁症模型。造模结束后通 过大鼠行为学指标来检测造模是否成功，剔除不合格大鼠。造模成功后开 始给药，正常对照组继续每日给予正常饮食及水，模型组每天 2ml 生理 盐水灌胃，氟西汀组以 10%阿拉伯凝胶制成混悬液，按照每日 2mg/kg 灌 胃给药，每日一次，连续 21 天，化浊解毒疏肝方高、低剂量组将人与大 鼠按照体表面积换算后按生药量 11.4g/kg·d, 2.75g/kg·d 给药,柴胡疏肝散 按生药量 3.85 g/kg·d 灌胃给药。给予每只大鼠预先定量好的两瓶水：1 瓶 1%蔗糖水，1 瓶纯水，观察 24h 内糖水和纯水消耗，糖水偏好=糖水消耗 /总液体消耗×100%。每日给予大鼠 100g 鼠粮，在造模前，造模后，治疗 后观察大鼠体重变化，造模结束后测糖水偏好，敞箱实验；治疗后，所有 大鼠依次进行敞箱实验、Y 迷宫、抬高实验和 Morris 水迷宫实验。每组 大鼠禁食 24h 处死，用免疫组化法测量大鼠大脑海马区神经肽 Y 的含量。 结果：1 大鼠体重变化：造模前，各组大鼠体重比较，经多因素方 差分析统计