环境低温诱发肉鸡肺血管重塑的细胞生物学机制研究-基础兽医学专业论文.docxVIP

重要因素，这两方面是环境低温诱发肉鸡肺血管重塑的主要细胞生物学机制。 关键词：肉鸡肺动脉高压综合征 环境低温 细胞增殖原癌基因 肉鸡 II  Abstract Pulmonary vascular remodeling is characterized by thickening media in pulmonary arteries，which is an important pathological feature of pulmonary hypertension syndrome(PHS)in broiler chickens， greatly contributes to a sustained elevation of pulmonary arterial pressure and has all important role in the development of PHS in broiler chickens．It is suggested that the thickened media may be the result of the increased the amount of vascular smooth muscle cells because of the proliferation which is motivated by abnormal expression of proto·oncogene in mammals．So we dynamically examined the characters of pulmonary vascular remodeling，proliferation of vascular smooth muscle cells，the expression of c-fos and c-jun in vascular smooth muscle cells in vivo in the development of PHS in broiler chickens induced by low ambient temperature，and the effect of calcium antagonist—verapamil on the above indexes，to provide the basis for the mechanism of the PHS． 1．Mean pulmonary artery pressure(mPAP)，packed cell volume(PCV)，the ratio of the weight of the right ventricle to the total ventricular weight of the hean(RV：TV)and the incidence of PHS of broilers were determined in the development of PHS．The results showed：(1)mPAP，PCV and RV：TV of broilers in low temperature group were separately increased from 22，29 and 29 to 50 days of age compared with those in the control group and verapamil group．2、The incidence of PHS in low temperature group Was significantly higher than that in the control group and verapamil group伊O．0 1)． The results suggested that PHS Was successfully induced by low ambient temperature and verapamil prevented the development of PHS． 2．Lung sections were stained with Weight and counterstained with Van Gieson to determine the pulmonary vascular remodeling by using the image analyzing system．The results showed：(1)Relative medial thickness(RMT％)and Relative lumen area(RLA％)of pulmonary arteri