课件：医学寄生虫——阿米巴鞭毛虫.pptx

Amoeba and flagella 阿米巴和鞭毛虫 . Entamoeba histolytica (E. histolytica) 溶组织内阿米巴 . Giardia lamblia (G. lamblia)　兰氏贾第鞭毛虫 . Trichomonas vaginalis (T. vaginalis)　阴道毛滴毛虫 . Leishmania donovani (L. donovani)　杜氏利什曼原虫Purpose and Requirements .Master morphological features of trophozoite and cyst of Entamoeba histolytica and Giardia lamblia; Master movement characteristics of trophozoites; .Master morphological features and movement characteristics of Trichomonas vaginalis; .Familiar with differtiations of E. histolytica cyst and other non-pathogenic amoebae cyst; .Familiar with iodine stain for cyst; .Understanding Balantidium coli; .Understanding morphological features of amastigote of Leishmania donovani and sandfly. .　本次课学习任务　1.独立观察玻片标本 No.原-1至No.原-4：　2.示教内容学习： 1）图片及视频示教： 2）镜下示教标本：　3.完成实验操作：　①直接涂片法观察活动的阿米巴滋养体；　②直接涂片法观察活动的阴道毛滴虫；　③碘染色法观察包囊 4.完成实验报告：　　 1.Observation of glass slide No.原-1至No.原-4　拿到玻片标本后请马上检查核对是否有漏、重复或破损，有问题的请马上举手报告老师！　No.原-1: 溶组织内阿米巴包囊、结肠内阿米巴包囊、微小内蜓阿米巴包囊、布氏嗜碘阿米巴包囊　Cyst of E. histolytica, E. coli, E. nana, I. buetschlii　No. 原-2: 蓝氏贾第鞭毛虫滋养体 G. lamblia trophozoite (仅双号同学有，2个同学共用1张)　No. 原-3: 蓝氏贾第鞭毛虫包囊 G. lamblia cyst　No. 原-4: 阴道毛滴虫滋养体 T. vaginalis trophozoite 注意：①在观察玻片标本前，先将显微镜光线调到最强！　②本次课只使用10倍镜和油镜； 1.Observation of glass slide No.原-1至No.原-4 No. 原-4:阴道毛滴虫滋养体T. vaginalis trophozoite先10倍镜下调出焦距平面，然后转油镜下观察细微结构要求拍照Nucleus4 anterior flagellum 4根前鞭毛Lateral flagellum and Undulating membrane 波动膜 Axostyle 轴柱姬姆萨染色法(Giemsa staining method) 1.Observation of glass slide No.原-1至No.原-4 No. 原-3:蓝氏贾第鞭毛虫包囊 G. lamblia cyst先10倍镜下调出焦距平面，然后转油镜下观察细微结构要求拍照. Cyst wall : . Axostyle : . Median body : . Nucleus : Cyst wall 囊壁Nucleus 核Axostyle 轴柱Median body 中体Steined with iron-hematoxylin 1.Observation of glass slide No.原-1至No.原-4 No. 原-2:蓝氏贾第鞭毛虫滋养体 　G. lamblia trophozoite先10倍镜下调出焦距平面，然后转油镜下观察细微结构要求拍照Adhesive disc 吸盘Anterior flagellum 前鞭毛NucleusMedian body 中体Ventral flagellum 腹鞭毛Posterior flagellum 后鞭毛Axostyle 轴柱Caudal flagellum 尾鞭毛姬姆萨染色法(Giemsa staining method) 1.Observation of glass