ArcA蛋介导的大肠杆菌细胞周期调控-生物化学与分子生物学专业毕业论文.docxVIP

Arc．A蛋白介导的大肠杆菌细胞周期调控ARCA．-MEDL～TED Arc．A蛋白介导的大肠杆菌细胞周期调控 ARCA．-MEDL～TED RE(矾Ⅱ．，ATION OF T胍 E贮H邑!R，C删C(Ⅺ■CELL CYCLE AB STRACT The Escherichia coli ArcB／ArcA two-component system is important signaling system，responding to anaerobic condition．However，we found that ArcA also affects cell cycle under aerobic condition． The study found that mutant of the arcA gene delayed initiation of DNA replication，decreased cell size and growth rate in LB medium；the mutant of area led to an early initiation of replication and larger cells in ABTGcasa medium．The results indicate that the effects of ArcA on initiation of replication and cell division depend on nutrition．Interestingly，mutant of the arcB gene also affected initiation of replication in the manner of ArcA—mediated effects，suggesting that the ArcB／ArcA signal pathway affects initiation of replication．Western blot analysis showed that mutant of arcA led to a decrease in DnaA amount per cell grown in LB while an increase in ABTGcasa medium．Further，extemal addition of cysteine reversed the changes in initiation of replication caused by the mutant of arcA in mode of nutrition—dependent．It was found that ArcA-GFP colocalized with chromosomal 2 万方数据 内蒙古大学硕士学位论文DNA 内蒙古大学硕士学位论文 DNA m LB medium while did not in ABTGcasa medium． We concluded that ArcA affects initiation of DNA replication by changing amount of DnaA per cell，the effect depends on nutrition． KEYWORDS：ArcA；replication initiation；DnaA；cell cycle；cysteine 3 万方数据 Arc．．A蛋白介导的大肠杆菌细胞周期调控目录 Arc．．A蛋白介导的大肠杆菌细胞周期调控 目录 一、大肠杆菌ArcB／ArcA信号转导系统应答无氧环境(文献综述) ～7 1．1感知蛋白mcB的激活 7 1．2应答调节蛋白～cA的存在形式及其激活 9 1．2．1 AreA的存在形式 9 1．2．2 ArcA的激活 ．9 1．3 ArcA的多效性调控 ．10 1．3．1 AreA调控物质及能量代谢相关基因的表达 ．10 1．3．2 ArcA调控大肠杆菌细胞周期 ．1l 1．3．3 ArcA参与细胞对ROS的耐受调节 ll 1．3．4 ArcA影响细菌运动 ．12 1．3．5 ArcB／ArcA信号系统调控转运蛋白的表达 12 1．3．6 AreA对细胞内重要转录因子的表达调控 ．12 1．3．7 ArcB／ArcA信号系统参与膜压力改变的调控 12 1．4 ArcA调控基因表达的特点 13 1．5 ArcB／ArcA信号转导系统与无氧应答蛋白Fnr之间的联系与区别 ．．13 二、ArcA蛋白调控大肠杆菌细胞周期 。14 2．1引言 ．．14 2．1．1 DNA复制起始的调控 ．14 2．1．2ArcB／ArcA信号转导系统调控