红曲霉发酵合成洛伐他汀的分析-食品工程专业论文.docxVIP

Abstract Lovastatin, a secondary metabolite of some Monascus, is a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, thereby it has effective functions to decrease blood sugar and blood fat. Monascus H8, the strain with the highest yield of Lovastatin, was screened from several strains. HPLC-MS, a method for structure identification of Lovastatin in the culture of Monascus H8, was adopted in this thesis and the result showed that the whole Lovastatin in the culture of Monascus H8 was Lovastatin hydroxy acid. The standard substance of Lovastatin lactone was hydrolyzed to Lovastatin hydroxy acid by means of alkaline hydrolysis, with the conversion ratio of 91.6%. Shaking-flask fermentation kinetics model of Monascus H8 as the test strain was constructed. Logistic equation was proposed for growth, Luedeking-Piret equation for Lovastatin biosynthesis and Luedeking-Piret-like equation for substrate consumption. The kinetic model provided a reasonable description for variations of biomass, product and substrate consumption along with the fermentation process. The best fermentation condition was obtained based on the single factor test and the multivariate orthogonal polynomial regression model. It showed the optimum medium compositions were: glycerol (60 mL/L), NaNO3 (3 g/L), soybean powder (3 g/L), K2HPO4 (1 g/L), MgSO4·7H2O (0.5 g/L), KCl (0.5 g/L), FeSO4·7H2O (0.01 g/L) and pH 5.0. The optimum technological parameters were as follows: culture temperature, 28.43℃; inoculation size, 10.10%; dissolved oxygen, 96.7%; fermentation time, 15 d. Under these culturing conditions, Lovastatin productivity of liquid fermentation was 142.51 mg/L. Biosynthesis of Monascus pigment was closely related to biosynthesis of Lovastatin. It was indicated that the productivity of Monascus pigment from Monascus H8 were improved with the increase of temperature and inoculation to some extent. But the yield of Lovastatin was a sharply fa