红曲霉发酵及某些生理活性物质的研究-生物化工专业论文.docxVIP

浙江工业大学硕士学位论文 浙江工业大学硕士学位论文 摘要 第1页 红曲霉发酵及某些生理活性物质的研究 摘 要 红曲霉所代谢的生理活性物质除Monacotins外，尚含有麦角甾醇 (Ergoster01)、1，一氨基丁酸(GAaA)、多糖等，均以引起国内外学者的极 大兴趣。本论文的主要目的是优化红曲霉发酵产Ergosterol、GABA的 工艺，并初步探讨Monascuspilosus GMl00规模化生产。 本研究分别筛选了高产Ergosterol及GABA的菌株Monascus ruber GM088及^如”∞c螂pilosus GMl00作为试验对象。对红曲霉发酵样品 中Ergosterol的测定进行了紫外分光光度法和HPLC法探讨， 亦对 GABA测定进行了HPLC．ELSD法及氨基酸自动分析仪法的研究。 分别优化了试验红曲霉菌株固体和液体发酵产Ergosterol及GABA 的条件。在本试验较优工艺条件下，液体发酵Ergosterol单位含量达 3．249／1 009干菌体，GABA的含量为38．5I_tg／mh固体发酵Ergosterol 单位含量为3．86mg／g，GABA的含量为330mg／kg。 采用100L发酵罐及通风曲池对Monascus pilosus GMl00菌株产 GABA进行扩大培养，实验表明发酵滤液的GABA含量达1 801jg／ml， 发酵液菌体中GABA含量为7101Ag／g；通风曲池生产的红曲米GABA 的含量达390Atg／g，经C02 48h厌氧处理，提高到4701ag／g。 关键词：红曲霉，Ergosterol，GABA，固液体发酵，通风制曲 塑望三些奎堂堡主兰垡望苎 塑望三些奎堂堡主兰垡望苎 堂!坐!! 塑!二!亘 RESEARCH OF SOM匣PHYSIOLOGICAL ACTIVE SUBSTANCE BY FERA伍N，I：ATION OF MONASCUS JS!f垆 ABSTRACT Many researchers at home and abmad have being highly interesting in some physiological active substances such as T-aminobutyric acid(GABA)，ergosterol， polysacchadde and so on except for Moncolins．The main purpose of this thews is to study the fermentation technology of ergosterol and GABA by Monascus spp．and researchlarge scaleproductionbyMonascuspilosusGMl00 strain． We screened Monascus ruber GM088 and Monascus pilosus GMl 00 strains which had respectively higher yield of ergosterol and GABA to be the object strains for our study．The UV spectrophtometric and HPLC methods which determine the content of ergosterol in the fermentation sample of Monascus are studied．We also study the HPLC-ELSD and amio acid analyzer methods which determine the content ofGABAin the fermentation sample ofmonascus are studied。 The optimal conditions for liquid and solid·state fermentation of ergosterol production by Monascus ruber GM099 and GABA production by Monascus GMl00 strain．Furthermore we study GABA large scale production by fermentation of Monascus pilosus GMl00．In the optimal conditions the content of ergosterol is 3．249／1 009 dry mycelium by liquid fermenta