miR183负调控下游靶基因PTPN4促进肺腺癌始动细胞侵袭迁移作用研究.docx

第三军医大学博士学位论文 Reaction Tris Tris(hydroxylmethyl)aminomethane 三羟甲基氨基甲烷 CCK8 Cell Counting Kit一8 细胞计数试剂盒 Real time PCR Real time Polymerase Chain Reaction 实时定量PCR 0D Optical Density 光密度 TBST Tris Buffered Saline with Tween 20 TBST缓冲液 rpm Revolutions per minute 姨|食 ‘ CY3 Cyanine 3 青蓝3荧光素 EB ethidium bromide 溴化乙锭 EP—CAM epithelial cell adhesion molecule 上皮细胞粘附分子 PAGE Polyacrylamide Gel Electrophoresis 聚丙烯酰胺凝胶电泳 TICs Tumour initiating cells 肿瘤始动细胞 PTPN4 protein tyrosine phosphatase non-receptor 蛋白酪氨酸磷酸化非受体4 type4 SP Side Population Cells 侧群细胞 PCR Polymerase Chain Reaction 聚合酶链反应 RAB member RAS oncogene family RAS癌基因家族 PDCD4 programmed cell death 4 程序性细胞死亡蛋白4 TEMED tetramethylethylenediamine 四甲基乙二胺 2 万方数据  第三军医大学博士学位论文 MicroRNA-183 promotes motility of CDl33+／CD326+ CSLCs through PTPN4 repression Abstract Background Non·small cell lung cancer(NSCLC)is one of the most common cancers worldwide and ranks aS a leading cause of lung cancer mortality partly due to distant metastasis even in the early stages．An increasing number of studies have revealed that primary tumors contain a rare subpopulation of cells，termed cancer stem-like cells(CSLCs)or tumor initiating cells(TICs) that possess stronger invasiveness to form metastases in remote organs． MicroRNAs(miRNAs)are a class of small，endogenous，non—coding RNAs that negatively regulate gene expression by partial or entire complementary binding to 3untranslatedregion(UT鼬of mRNAs．Emerging evidence reveals that abnormal miRNA expression is relevant to the dysregulation of CSLCs in various cancers such as miR一1 8 1 and hepatic CSLCs，miR34 and pancreatic CSLCs，miR-340 and glioma CSLCs，miR一125a and breast CSLCs，miR-17 and ovarian CSLCs，miR-146a and colorectal CSLCs，and miR-320 and prostate CSLCs． Previous studies revealed that miR一1 83 Can mediate the invasiveness and growth of NSCLC．However,the exact role ofmiR·183 in regulating the biological behavior ofCSLCs in NSCLC remains unclear???Thus，a better recognition of abberant miR一1