广西草珊瑚种质资源遗传多样性的AFLP分析.PDFVIP

• 1398 • 中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 7 期 2012 年 7 月 广西草珊瑚种质资源遗传多样性的 AFLP 分析 唐美琼 1, 2，韦荣昌 1, 2, 3，姚绍嫦 1, 2，蓝祖栽 1, 2* ，凌征柱 1, 2 1. 广西药用植物园，广西 南宁 530023 2. 广西药用资源保护与遗传改良重点实验室，广西 南宁 530023 3. 中国医学科学院北京协和医学院 药用植物研究所，北京 100193 摘 要：目的 探索广西草珊瑚 Sarcandra glabra 种质资源的遗传多样性，为草珊瑚及其他中药种质资源保护、亲缘关系研 究、品种选育和引种栽培提供分子生物学依据。方法 利用 AFLP 标记对来自广西不同产地的 18 份草珊瑚开展遗传多样性 分析，采用 NTSYS 软件进行聚类和主坐标分析。结果 从 64 对 AFLP 引物组合中筛选出 8 对用于扩增，共获得 319 条清 晰可辨条带，其中多态性条带 251 条，多态性位点百分率为 78.68% 。聚类和主坐标分析表明广西草珊瑚种质资源的遗传基 础存在一定的多样性，但遗传相似性系数（0.630 3～0.836 6 ）较大，遗传距离较近。结论 广西草珊瑚种质资源的遗传多样 性偏低，应该尽快采取相应措施，对草珊瑚资源进行合理保护。 关键词：草珊瑚；种质资源；遗传多样性；AFLP ；多态性条带 中图分类号：R282.23 文献标志码：A 文章编号：0253 - 2670(2012)07 - 1398 - 05 AFLP analysis on genetic diversity of Sarcandra glabra in Guangxi region TANG Mei-qiong1, 2, WEI Rong-chang1, 2, 3, YAO Shao-chang1, 2, LAN Zu-zai1, 2, LING Zheng-zhu1, 2 1. Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China 2. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Nanning 530023, China 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China Abstract: Objective To evaluate the genetic diversity of germphlasm resources for Sarcandra glabra and provide biological reference for germplasm resources protection, genetic relationship analysis, breeding, and introduction and cultivation of S. glabra and other Chinese herbal medicines. Methods Amplified fragment length polymorphism (AFLP) marker was developed to analyze the genetic diversity among 18 samples of S. glabra from various habitats in Guangxi region, and cluster analysis and principal coordinate analysis were revealed by the NTSYS software. Results Eight out of sixty-four AFLP primer combinations selected were used for amplification and a total of 319 unambiguous bands were obtained, among whi