钝齿棒杆菌产精氨酸关键酶分析.pdfVIP

※基础研究 食品科学 2005, Vol. 26, No. 3 35 钝齿棒杆菌产精氨酸关键酶分析 陈雪岚，许正宏，陶文沂 (江南大学教育部工业生物技术重点实验室，江苏无锡 2 14036) 　　　　　　　 摘 要：采用亲和层析和SDS方法分析钝齿棒杆菌产精氨酸关键酶。实验表明此菌产精氨酸关键酶之一是 乙酰谷氨酸激酶，其分子量为34k D ；同时另一分子量为4 9kD 的蛋白质亦对精氨酸有较高的亲和性，揭示钝齿棒 杆菌产精氨酸的代谢途径中受精氨酸抑制的酶不是唯一的。实验对限速酶之一——乙酰谷氨酸激酶的酶学性质做了 初步研究，结果显示，此酶的最适反应温度为4 9 ℃，最适p H 为8 .0 ；金属离子P b 2 + 、Cu 2 + 、M n 2 + 和F e2 + 对乙 酰谷氨酸激酶有强烈的抑制作用；D T T 对该酶的活力有一定的影响。 关键词：钝齿棒杆菌；精氨酸；关键酶 Analysis the Key Enzymes Producing Arginine from Corynebacterium crenatum CHEN Xue-lan ，XU Zheng-hong ，TAO Wen-yi (Key Laboratory of Industry Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 2 14036, China) Abstract ：The key enzymes producing arginine from Cory nebavterium crenatum were investigated by the methods of affinity and SDS. The results indicated that one of the key enzymes was acetylglutamate kinase with around 34 kD. estimated by SDS, and the other protein with around 49kD. estimated by SDS, showed the affinity to arginine. The above mentioned results indicated that the key enzymes, restrained by arginine in arginine metabolize path of Corynebacterium crenatum, were not the only one. The properties of acetylglutamate kinase were also primarily studied. The results indicated that the optimum reaction temperature was 49 ℃and optimum pH 8.0. The protease activity of the enzyme was strongly inhibited by Pb2+ 、Cu2+ 、Mn2+ and Fe2+ , and was affected definitely by DTT. Key words ：Cory nebavterium crenatum ；arginine ；key enzyme 中图分类号：Q55 文献标识码：A 文章编号：1002-6630(2005)03-0035-05 精氨酸是生物体尿素循环的一种重要中间代谢产 酰基团方式的不同，L -Arg 的代