REV和CAV共感染的检测3.PDFVIP

、 我国自然发病鸡群中 MD V REV 和 CAV 共感染的检测 1 1 1 2 1 姜世金 ,孟珊珊 ,崔治中 , 田夫林 ,王增福 ( 1. 山东农业大学动物科技学院 , 山东泰安 27 10 18 ; 2 . 山东省畜牧兽医检验中心 , 山东济南 250022) Epidemic Investigation of Coinf ection of MD V , CAV and REV in Spontaneous Diseased Chicken Flocks in China J IAN G Shij in1 ,Meng Shanshan1 ,CU I Zhizhon g1 , T IAN Fulin2 ,WAN G Zengf u1 ( 1. A ni m al S cience an d Technol ogy Col leg e , S han dong A g ricul t u re Uni vers ity , Tai an S han dong 27 10 18 ; 2 . S han dong H us ban d ry an d Veteri nary i nsp ect ion center , J i nan S han dong 250022) Abstract : The ti ssue s samp le s of 828 chicken s su sp ect ed to be inf ect ed by immuno dep re ssive vir u se s f ro m 42 shicken flock s were collect ed in Shando ng , Henan , Hebei ,Beij in g ,J ian gsu , Guan g dong , Guangxi ,Sichuan ,J ilin ,Liao ning and Taiwan ,an d t he DN A wa s ext ract ed for hybridizatio n in Dotblot wit h p robe s for Marek ′s di sea se vir u s (MDV) , Reticuloendot helio si s vir u s ( R EV) an d Chicken anemia vir u s (CA V) . The re sult s in dicat ed t hat t he checkout ratio s of t he t hree vir u se s are 83 . 94 % ( MDV ) ,6 1. 0 % ( CA V ) and 57 . 25 % ( R EV ) ;t he ratio s of t rip le inf ection , dual inf ec tion ,sin gle inf ection and negative are 44 . 69 % ,3 1. 16 % , 15 . 34 % and 8 . 82 % ; re sp ectively ;29 of 42 chicken flock s occur t rip le inf ectio n (69 . 05 %) ,5 one s occur dual inf ectio n of MDV an d CAV ( ) ( ) 11. 90 % 3 one s