正交试验优化苦豆子多糖的双氧水脱色工艺研究.PDF

·1234 · 现代药物与临床 Drugs Clinic 第 29 卷 第 11 期 2014 年 11 月 正交试验优化苦豆子多糖的双氧水脱色工艺研究 王文彤，张 岩，陶遵威 天津市医药科学研究所，天津 300020 摘 要：目的 优化苦豆子多糖的脱色条件。方法 以多糖脱色率和多糖保留率为指标，考察双氧水（H O ）对苦豆子多 2 2 糖溶液的脱色效果。通过单因素试验考察 H2O2 脱色方法中双氧水加入量、脱色时间、脱色温度和 pH 值对苦豆子多糖脱色 效果的影响，进一步采用正交试验确定苦豆子多糖脱色的最佳条件。结果 H2O2 对苦豆子多糖最佳脱色条件为脱色温度 60 ℃，调节pH 5 ，加入 1.0%的H O ，脱色反应 3 h 。在此条件下经验证，多糖脱色率和多糖保留率可分别达到29.83% 、 2 2 71.12% 。结论 H2O2 脱色具有较好的脱色效果，可为生产出高品质的苦豆子多糖提供依据。 关键词：苦豆子多糖；脱色；双氧水；正交试验 中图分类号：R284.2 文献标志码：A 文章编号：1674 - 5515(2014)11 - 1234 - 04 DOI: 10.7501/j.issn.1674-5515.2014.11.010 Optimization of decolorization technology of Sophora alopecuroides polysaccharide with H O by orthogonal test 2 2 WANG Wen-tong, ZHANG Yan, TAO Zun-wei Tianjin Institute of Medical Sciences, Tianjin 300020, China Abstract: Objective To optimize the decolorization conditions on Sophora alopecuroides polysaccharide. Methods The effects of H2O2 on decolorization of S. alopecuroides polysaccharide were studied with polysaccharide decolorization rate and polysaccharide retention rate as indexes. The amount of H O , bleaching time, bleaching temperature, and pH value were studied through single factor 2 2 test in H O bleaching method, and the further optimal conditions of S. alopecuroides polysaccharide were studied by orthogonal test. 2 2 Results The optimal decoloring conditions for S. alopecuroides polysaccharide with H O were as following: decolorization 2 2 temperature of 60 ℃, pH value of 5, addition of 1% H O , and decolorization reaction for 3