先进固体发酵技术ASSF生产甜高粱乙醇-微生物学报.PDF

生 物 工 程 学 报 Chin J Biotech 2010, July 25; 26(7): 966–973 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 研究报告 先进固体发酵技术 (ASSF) 生产甜高粱乙醇 韩冰，王莉，李十中，王二强，仉磊，李天成 清华大学核能与新能源技术研究院新能源所，北京 100084 摘 要: 介绍了利用高产能源作物甜高粱生产燃料乙醇的先进固态发酵 (ASSF) 技术，从甜高粱茎秆保存、菌种、反应 器，到固体发酵过程的数学模拟和工程放大进行了系统研究。筛选出高效产乙醇的菌种CGMCC1949 ，固体发酵时间低于 30 h ，乙醇收率高于92 ％；优选出贮存甜高粱茎秆的有效方法，通过抑菌处理，厌氧贮存200 d 糖分损失小于5 ％；对固 态发酵过程进行了数学模拟，设计并优化了固体发酵设备，成功进行了工程放大试验，并且基于ASPEN 软件对该技术进 行了技术经济评价，结果表明ASSF 法生产甜高粱乙醇在技术、工程和经济上均具有充分的可行性和明显优势。 关键词: 甜高粱，先进固态发酵，燃料乙醇 Ethanol production from sweet sorghum stalks by advanced solid state fermentation (ASSF) technology Bing Han, Li Wang, Shizhong Li, Erqiang Wang, Lei Zhang, and Tiancheng Li Institute of Nuclear and New Energy Technology , Tsinghua University, Beijing 100084, China Abstract: A robust strain of the species Saccharomyces cerevisiae CGMCC1949 was screened and identified, and advanced solid state fermentation (ASSF) technology for fuel ethanol production from sweet sorghum stalks was thus developed. The fermentation time was shortened to less than 30 h, and ethanol yield was 92% of its theoretical maximum. And in the meantime, the cost-effective storage was established for sweet sorghum stalks, with less than 5% sugar loss after 200 days of storage, making the plant operation could extend up to 200 days without feedstock shortage. With the fermentation kinetics and heat-mass transfer models, modeling of the ASSF process was investigated, and the rotating drum bioreactor was designed. Furthermore, the ASSF