铜氨法生产叶绿素铜钠盐工艺优化.PDF

※工艺技术 食品科学 2012, Vol. 33, No. 12 119 喻 薇，纪平雄 * (华南农业大学理学院 生物材料研究所，广东 广州 510642) 叶绿素铜钠盐作为一种安全无毒的水溶性叶绿素衍生物，具有良好的稳定性和着色性，被广泛应用于食 品添加剂。本研究对以蚕沙叶绿素为原料制备叶绿素铜钠盐的传统工艺进行了改进，以碱性铜氨置换法半合成叶绿 素铜钠盐。选择铜代过程中乙醇质量分数、pH 值、反应温度及加铜量 4 个试验因素，通过正交试验和 SAS 统计 软件进行优化分析，得出最适工艺条件为加铜量为理论值的 2.5 倍、乙醇质量分数 75% 、温度55 ℃、pH11.5 。减 少置铜过程溶剂乙醇用量 10%，得到的产品质量符合国家规定食品添加剂标准。 蚕沙；铜氨法；叶绿素铜钠盐；工艺条件；S A S 统计 Optimization of Synthesis of Sodium Copper Chlorophyllin by Alkaline Cuprammonium Replacement YU Wei ，JI Ping-xiong* (Institute of Biomaterial, College of Science, South China Agricultural University, Guangzhou 510642, China) Abstract ：Chlorophyll copper sodium salt as a non-toxic water soluble chlorophyll derivative has excellent stability and colorability and is widely used as a food additive. In the present study, the traditional preparation process for sodium copper chlorophyllin from silkworm excrement chlorophyll was modified to develop a semi-synthesis method based on alkaline cuprammonium replacement. Four reaction conditions such as ethanol concentration, pH, reaction temperature and copper amount were chosen for synthesis process optimization based on orthogonal array design using SAS statistical software. The optimal copper amount, ethanol concentration, reaction temperature and pH were 2.5 times higher than the theoretic value, 75%, 55 ℃, and 11.5, respectively. Under these conditions, the ethanol consumption for copper replacement was reduced by 10%, and the quality of the product obtained was in accordance with the national standard of food additives. Key words ：silkworm excrement ；cuprammonium method ；sodium copper chlorophyllin ；process conditions ；SAS statistical so