解偶联蛋白2通过线粒体介导参与高血糖加重脑缺血再灌注损伤的实验分析.pdfVIP

宁夏医科大学硕士研究生论文 英文摘要 Uncoupling protein 2 play a role in hyperglycemia aggravated cerebral ischemia-reperfusion injury mediated by the mitochondria ABSTRACT Objective Diabetes hyperglycemia aggravated cerebral ischemia - reperfusion injury,and promoted production of mitochondrial reactive oxygen species (ROS) after ischemia. Uncoupling protein 2 (UCP2) can stabilized the mitochondrial membrane potential and re duced the generation of ROS.But at present, mechanism of action and regulatory pathway of UCP2 in hyperglycemia aggravated cerebral ischemia injury are not clear.This study using UCP2 knockout mice simulated cerebral ischemia and hyperglycemia ，to explore i f UCP2 knockout can aggravated neuronal injury and its molecular pathways. Methods Male UCP2 knockout mice (KO -UCP2) and wild -type C57BL/6j mice were randomly divided into wild -type mice with normoglycemic focal cerebral I/R group(CNgroup), KO -UCP2 mice w ith normoglycemic focal cerebral I/R group(KN group) and KO-UCP2 mice with diab etic hyperglycemic I/R group(KHgroup).A type Ⅰdiabetic rat model was induced by streptozotocin (STZ) injection and the focal cerebral I/R model was induced by 1 hour of middle cerebral artery occlusion (MCAO) followed by6h,24h,72h of reperfusion.Histopa - thology,immunohistochemistry,immunofluorescence,TTCstaining,TUNEL,RT-PC Rand western blot were applied to detect changes of the morphology and the number of neurons, apoptosis and th e expression of Cyt