基本原理及应用.pptVIP

 ABI PRISM? 7900HTSequence Detection System 基本原理及应用 美国应用生物系统中国公司维修部2002.6 Basic Arithmetic Formula Where: Rn=Total fluorescence signal RB=Base fluorescence signal XO=Initial number of target molecules EX=Efficiency of target amplification n =Number of cycle RS=Fluorescence signal per target molecule For Specific PCR Reaction RB, Rs and Ex is constant When fluorescence reach threshold, Rn=RT=Threshold is constant, n=CT= threshold cycle number of target molecules so that SYBR? Green分解曲线 TaqMan?和SYBR?Green的区别 TaqMan?/TaqMan? MGB化学 基于引物和探针的高度特异性 SYBR?Green化学 基于引物的特异性 较TaqMan?便宜，用于对特异性