大气NO浓度升高增强植物抗盐胁迫能力的研究.docVIP

PAGE 大气NO浓度升高增强植物抗盐胁迫能力的研究 摘 要：土壤盐渍化对现代农业生产来说是面临的最主要问题之一。盐胁迫会使植物的生长发育受阻，寻找一些适当的措施来减缓这种胁迫是非常迫切的。由于NO供体能缓解盐胁迫的一些症状，但是不是以NO气体的形式也能增强植物的抗盐胁迫能力尚未清楚。因此，本实验运用了营养水培的方法，研究了外源气体一氧化氮（NO，200 nL L-1）对0或者200 mmol L-1 NaCl胁迫下菠菜幼苗生长情况及抗氧化胁迫的影响。结果表明，外源NO气体能使盐胁迫菠菜地上部分的鲜重和干重分别增加了60%和27.3%，地下部分的鲜重和干重分别增加了81.8%和45.9%。叶绿素含量和光合作用分别增加25.6%和74.9%。抗氧化胁迫指标MAD降低18.5%，H2O2含量下降20%。上述结果说明了使用微量的NO气体是能够减缓盐胁迫的逆境反应促进产量的增加。 关键词：盐胁迫，NO气体，抗盐，菠菜 Trace amount of atmospheric NO can alleviate salt stress in plant Abstract: Soil salinization is one of the main problem in agricultural production. The plants growth and development can be inhibited under salt stress. So It is very urgent to find some proper measures to alleviate the salt stress. Due to the NO donor can alleviate the symptoms of salt stress, whether the capacity of resistance to salt stress in plants could be enhanced by treated with NO gas is still unclear. Therefore, the present study was conducted in nutrient solution of 0 or 200 of NaCl by atmospheric application of 200 nL L-1 NO gas in spinach seedlings. The results showed that exogenous NO can significantly restore the damage of spinach seedlings under NaCl stress with fresh and dry biomasses increased by 60% and 27.3%, respectively. Compared with the control, gaseous NO application also elevated the fresh and dry roots by 81.8% and 81.8% respectively. Furthermore, the chlorophyll content and photosynthetic rate were increased by 25.6% and 74.9% in NO treatment, respectively. Meanwhile, the antioxidant stress index MAD and H2O2 content were reduced by 18.5% and 20% in NO treatment, respectively. Therefore, we concluded that atmospheric application of trace amounts of nitric oxide gas could be an effective and convenient strategy for alleviating salt stress and thereby improve biomass production.. Keywords: Salt stress, NO gas, resistance to salt, spinach  正文目录 TOC \o 1-2 \h \z \u HYPERLINK \l _Toc387436681 1引言 Ⅰ-1 HYPERLINK \l _Toc387436682 2实验方法 Ⅰ-3 HYPERLINK \l _Toc387436683 2.1